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  In Vivo Visualization of Cardiomyocyte Apicobasal Polarity Reveals Epithelial to Mesenchymal-like Transition during Cardiac Trabeculation

Jimenez-Amilburu, V., Rasouli, S. J., Staudt, D. W., Nakajima, H., Chiba, A., Mochizuki, N., et al. (2016). In Vivo Visualization of Cardiomyocyte Apicobasal Polarity Reveals Epithelial to Mesenchymal-like Transition during Cardiac Trabeculation. CELL REPORTS, 17(10), 2687-2699. doi:10.1016/j.celrep.2016.11.023.

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 Creators:
Jimenez-Amilburu, Vanesa1, Author              
Rasouli, S. Javad1, Author              
Staudt, David W., Author
Nakajima, Hiroyuki, Author
Chiba, Ayano, Author
Mochizuki, Naoki, Author
Stainier, Didier Y.R.1, Author              
Affiliations:
1Developmental Genetics, Max Planck Institute for Heart and Lung Research, Max Planck Society, ou_2591697              

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Free keywords: ASYMMETRIC CELL DIVISIONS; 2 DISTINCT STEPS; APICAL CONSTRICTION; HEART DEVELOPMENT; ZEBRAFISH HEART; DROSOPHILA NEUROBLASTS; NEUREGULIN RECEPTOR; CHAMBER MATURATION; DORSAL CLOSURE; C-ELEGANSCell Biology;
 Abstract: Despite great strides in understanding cardiac trabeculation, many mechanistic aspects remain unclear. To elucidate how cardiomyocyte shape changes are regulated during this process, we engineered transgenes to label their apical and basolateral membranes. Using these tools, we observed that compact-layer cardiomyocytes are clearly polarized while delaminating cardiomyocytes have lost their polarity. The apical transgene also enabled the imaging of cardiomyocyte apical constriction in real time. Furthermore, we found that Neuregulin signaling and blood flow/cardiac contractility are required for cardiomyocyte apical constriction and depolarization. Notably, we observed the activation of Notch signaling in cardiomyocytes adjacent to those undergoing apical constriction, and we showed that this activation is positively regulated by Neuregulin signaling. Inhibition of Notch signaling did not increase the percentage of cardiomyocytes undergoing apical constriction or of trabecular cardiomyocytes. These studies provide information about cardiomyocyte polarization and enhance our understanding of the complex mechanisms underlying ventricular morphogenesis and maturation.

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Language(s): eng - English
 Dates: 2016
 Publication Status: Published in print
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

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Title: CELL REPORTS
Source Genre: Journal
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Publ. Info: 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA : CELL PRESS
Pages: - Volume / Issue: 17 (10) Sequence Number: - Start / End Page: 2687 - 2699 Identifier: ISSN: 2211-1247