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  N-3 vs. n-6 fatty acids differentially influence calcium signalling and adhesion of inflammatory activated monocytes: impact of lipid rafts

Schaefer, M. B., Schaefer, C. A., Schifferings, S., Kuhlmann, C. R. W., Urban, A., Benscheid, U., et al. (2016). N-3 vs. n-6 fatty acids differentially influence calcium signalling and adhesion of inflammatory activated monocytes: impact of lipid rafts. INFLAMMATION RESEARCH, 65(11), 881-894. doi:10.1007/s00011-016-0971-9.

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 Creators:
Schaefer, Martina Barbara, Author
Schaefer, Christian Alexander, Author
Schifferings, Stefanie, Author
Kuhlmann, Christoph Ruediger Wolfram, Author
Urban, Annett, Author
Benscheid, Utz, Author
Fischer, Tobias, Author
Hecker, Matthias, Author
Morty, Rory E.1, Author           
Vadasz, Istvan, Author
Herold, Susanne, Author
Witzenrath, Martin, Author
Seeger, Werner1, Author           
Erdogan, Ali, Author
Mayer, Konstantin, Author
Affiliations:
1Lung Development and Remodeling, Max Planck Institute for Heart and Lung Research, Max Planck Society, ou_2591698              

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Free keywords: HUMAN ENDOTHELIAL-CELLS; DOCOSAHEXAENOIC ACID; EICOSAPENTAENOIC ACID; MYOCARDIAL-INFARCTION; MEMBRANE MICRODOMAINS; PARENTERAL-NUTRITION; B-CELLS; GENERATION; SEPSIS; MOLECULESCell Biology; Immunology; Lipid rafts; Calcium; Inflammation; Adhesion; Fatty acids;
 Abstract: Anti-inflammatory n-3 fatty acids (FA) like docosahexaenoic acid (DHA) opposed to the pro-inflammatory n-6 FA arachidonic acid (AA) might modulate lipid rafts within the cell membrane by differential incorporation. In inflammation, monocyte adhesion to endothelial cells is a crucial step mediated by intracellular calcium changes. We investigated whether lipid rafts mediate FA-induced modulation of adhesion and intracellular calcium. In isolated human monocytes and monocytic U937 cells we measured adhesion to human umbilical vein endothelial cells (HUVEC) using a parallel flow chamber and a static assay, adhesion molecules by FACScan, and intracellular calcium by fluorescence. Monocyte lipid rafts were isolated by ultracentrifugation and submitted to gas chromatography for FA analysis. Pre-incubation with AA or DHA resulted in a predominant incorporation of the respective FA into raft compared to non-raft fraction. DHA as compared to AA significantly reduced monocyte adhesion and calcium release after stimulation with TNF-alpha while expression of adhesion molecules remained unchanged. Pre-treatment with a calcium chelator abolished the effect of FA on calcium and adhesion. Disruption of lipid rafts prevented FA-induced modulations. Incorporation of FA into lipid rafts seem to be crucial for modulation of adhesion under inflammatory conditions.

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Language(s): eng - English
 Dates: 2016
 Publication Status: Published in print
 Pages: 14
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000385178300004
DOI: 10.1007/s00011-016-0971-9
 Degree: -

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Title: INFLAMMATION RESEARCH
Source Genre: Journal
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Publ. Info: PICASSOPLATZ 4, BASEL, 4052, SWITZERLAND : SPRINGER BASEL AG
Pages: - Volume / Issue: 65 (11) Sequence Number: - Start / End Page: 881 - 894 Identifier: ISSN: 1023-3830