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  N-cadherin relocalization during cardiac trabeculation

Cherian, A. V., Fukuda, N., Augustine, S. M., Maischein, H.-M., & Stainier, D. Y. (2016). N-cadherin relocalization during cardiac trabeculation. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 113(27), 7569-7574. doi:10.1073/pnas.1606385113.

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 Creators:
Cherian, Anoop V.1, Author              
Fukuda, Nana1, Author              
Augustine, Sruthy M.1, Author              
Maischein, Hans-Martin1, Author              
Stainier, Didier Y.R.1, Author              
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1Developmental Genetics, Max Planck Institute for Heart and Lung Research, Max Planck Society, ou_2591697              

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Free keywords: ADHERENS JUNCTIONS; IN-VIVO; CELLULAR MECHANISMS; NEUREGULIN RECEPTOR; FLUORESCENT PROTEIN; HEART DEVELOPMENT; ZEBRAFISH HEART; MIGRATION; ERBB2; MORPHOGENESISScience & Technology - Other Topics; N-cadherin; trabeculation; heart development; Erbb2 signaling; Cdh2-EGFP;
 Abstract: During cardiac trabeculation, cardiomyocytes delaminate from the outermost (compact) layer to form complex muscular structures known as trabeculae. As these cardiomyocytes delaminate, the remodeling of adhesion junctions must be tightly coordinated so cells can extrude from the compact layer while remaining in tight contact with their neighbors. In this study, we examined the distribution of N-cadherin (Cdh2) during cardiac trabeculation in zebrafish. By analyzing the localization of a Cdh2-EGFP fusion protein expressed under the control of the zebrafish cdh2 promoter, we initially observed Cdh2-EGFP expression along the lateral sides of embryonic cardiomyocytes, in an evenly distributed pattern, and with the occasional appearance of punctae. Within a few hours, Cdh2-EGFP distribution on the lateral sides of cardiomyocytes evolves into a clear punctate pattern as Cdh2-EGFP molecules outside the punctae cluster to increase the size of these aggregates. In addition, Cdh2-EGFP molecules also appear on the basal side of cardiomyocytes that remain in the compact layer. Delaminating cardiomyocytes accumulate Cdh2-EGFP on the surface facing the basal side of compact layer cardiomyocytes, thereby allowing tight adhesion between these layers. Importantly, we find that blood flow/cardiac contractility is required for the transition from an even distribution of Cdh2-EGFP to the formation of punctae. Furthermore, using time-lapse imaging of beating hearts in conjunction with a Cdh2 tandem fluorescent protein timer transgenic line, we observed that Cdh2-EGFP molecules appear to move from the lateral to the basal side of cardiomyocytes along the cell membrane, and that Erb-b2 receptor tyrosine kinase 2 (Erbb2) function is required for this relocalization.

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Language(s): eng - English
 Dates: 2016
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000379021700075
DOI: 10.1073/pnas.1606385113
 Degree: -

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Title: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Source Genre: Journal
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Publ. Info: 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA : NATL ACAD SCIENCES
Pages: - Volume / Issue: 113 (27) Sequence Number: - Start / End Page: 7569 - 7574 Identifier: ISSN: 0027-8424