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  HIPK family kinases bind and regulate the function of the CCR4-NOT complex

Rodriguez-Gil, A., Ritter, O., Hornung, J., Stekman, H., Krüger, M., Braun, T., et al. (2016). HIPK family kinases bind and regulate the function of the CCR4-NOT complex. MOLECULAR BIOLOGY OF THE CELL, 27(12), 1969-1980. doi:10.1091/mbc.E15-09-0629.

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 Creators:
Rodriguez-Gil, Alfonso, Author
Ritter, Olesja, Author
Hornung, Juliane, Author
Stekman, Hilda, Author
Krüger, Marcus1, Author              
Braun, Thomas1, Author              
Kremmer, Elisabeth, Author
Kracht, Michael, Author
Schmitz, M. Lienhard, Author
Affiliations:
1Cardiac Development and Remodeling, Max Planck Institute for Heart and Lung Research, Max Planck Society, ou_2591698              

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Free keywords: INTERACTING PROTEIN KINASE-2; RNA-POLYMERASE-II; NF-KAPPA-B; MESSENGER-RNA; SACCHAROMYCES-CEREVISIAE; DNA-DAMAGE; SUBCELLULAR-LOCALIZATION; TRANSLATIONAL REPRESSION; CATALYTIC-ACTIVITY; ACTIVATION-LOOPCell Biology;
 Abstract: The serine/threonine kinase HIPK2 functions as a regulator of developmental processes and as a signal integrator of a wide variety of stress signals, such as DNA damage, hypoxia, and reactive oxygen intermediates. Because the kinase is generated in a constitutively active form, its expression levels are restricted by a variety of different mechanisms. Here we identify the CCR4-NOT complex as a new regulator of HIPK2 abundance. Downregulation or knockout of the CCR4-NOT complex member CNOT2 leads to reduced HIPK2 protein levels without affecting the expression level of HIPK1 or HIPK3. A fraction of all HIPK family members associates with the CCR4-NOT components CNOT2 and CNOT3. HIPKs also phosphorylate the CCR4-NOT complex, a feature that is shared with their yeast progenitor kinase, YAK1. Functional assays reveal that HIPK2 and HIPK1 restrict CNOT2-dependent mRNA decay. HIPKs are well known regulators of transcription, but the mutual regulation between CCR4-NOT and HIPKs extends the regulatory potential of these kinases by enabling posttranscriptional gene regulation.

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Language(s): eng - English
 Dates: 2016
 Publication Status: Published in print
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000378096900010
DOI: 10.1091/mbc.E15-09-0629
 Degree: -

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Title: MOLECULAR BIOLOGY OF THE CELL
Source Genre: Journal
 Creator(s):
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Publ. Info: 8120 WOODMONT AVE, STE 750, BETHESDA, MD 20814-2755 USA : AMER SOC CELL BIOLOGY
Pages: - Volume / Issue: 27 (12) Sequence Number: - Start / End Page: 1969 - 1980 Identifier: ISSN: 1059-1524