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  Vegfa signaling promotes zebrafish intestinal vasculature development through endothelial cell migration from the posterior cardinal vein

Koenig, A. L., Baltrunaite, K., Bower, N. I., Rossi, A., Stainier, D. Y., Hogan, B. M., et al. (2016). Vegfa signaling promotes zebrafish intestinal vasculature development through endothelial cell migration from the posterior cardinal vein. DEVELOPMENTAL BIOLOGY, 411(1), 115-127. doi:10.1016/j.ydbio.2016.01.002.

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 Creators:
Koenig, Andrew L., Author
Baltrunaite, Kristina, Author
Bower, Neil I., Author
Rossi, Andrea1, Author              
Stainier, Didier Y.R.1, Author              
Hogan, Benjamin M., Author
Sumanas, Saulius, Author
Affiliations:
1Developmental Genetics, Max Planck Institute for Heart and Lung Research, Max Planck Society, ou_2591697              

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Free keywords: GROWTH-FACTOR-C; IN-VIVO; EMBRYONIC LYMPHANGIOGENESIS; LIVER ORGANOGENESIS; LYMPHATIC VESSELS; ANGIOGENESIS; VASCULOGENESIS; ARTERIAL; DIFFERENTIATION; ANGIOBLASTDevelopmental Biology; Zebrafish; Vegf; Intestinal; Vascular endothelial; Organ;
 Abstract: The mechanisms underlying organ vascularization are not well understood. The zebrafish intestinal vasculature forms early, is easily imaged using transgenic lines and in-situ hybridization, and develops in a stereotypical pattern thus making it an excellent model for investigating mechanisms of organ specific vascularization. Here, we demonstrate that the sub-intestinal vein (SIV) and supra-intestinal artery (SIA) form by a novel mechanism from angioblasts that migrate out of the posterior cardinal vein and coalesce to form the intestinal vasculature in an anterior to posterior wave with the SIA forming after the SIV. We show that vascular endothelial growth factor as (vegfaa) is expressed in the endoderm at the site where intestinal vessels form and therefore likely provides a guidance signal. Vegfa/Vegfr2 signaling is required for early intestinal vasculature development with mutation in vegfaa or loss of Vegfr2 homologs causing nearly complete inhibition of the formation of the intestinal vasculature. Vegfc and Vegfr3 function, however, are dispensable for intestinal vascularization. Interestingly, ubiquitous overexpression of Vegfc resulted in an overgrowth of the Sly, suggesting that Vegfc is sufficient to induce SIV development. These results argue that Vegfa signaling directs endothelial cells to migrate out of existing vasculature and coalesce to form the intestinal vessels. It is likely that a similar mechanism is utilized during vascularization of other organs. (C) 2016 Elsevier Inc. All rights reserved.

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Language(s): eng - English
 Dates: 2016
 Publication Status: Published in print
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

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Title: DEVELOPMENTAL BIOLOGY
Source Genre: Journal
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Publ. Info: 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA : ACADEMIC PRESS INC ELSEVIER SCIENCE
Pages: - Volume / Issue: 411 (1) Sequence Number: - Start / End Page: 115 - 127 Identifier: ISSN: 0012-1606