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  Correlations in Scattered X-Ray Laser Pulses Reveal Nanoscale Structural Features of Viruses

Kurta, R. P., Donatelli, J. J., Yoon, C. H., Berntsen, P., Bielecki, J., Daurer, B. J., et al. (2017). Correlations in Scattered X-Ray Laser Pulses Reveal Nanoscale Structural Features of Viruses. Physical Review Letters, 119(15): 158102. doi:10.1103/PhysRevLett.119.158102.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-C0F4-2 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-C0F5-1
Genre: Journal Article

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 Creators:
Kurta, R. P.1, Author
Donatelli, J. J.2, 3, Author
Yoon, C. H.4, Author
Berntsen, P.5, Author
Bielecki, J.1, 6, Author
Daurer, B. J.6, Author
DeMirci, H.7, 8, Author
Fromme, P.9, Author
Hantke, M. F.6, Author
Maia, F. R. N. C.6, 10, Author
Munke, A.6, Author
Nettelblad, C.6, 11, Author
Pande, K.3, 12, Author
Reddy, H. K. N.6, Author
Sellberg, J. A.6, 13, Author
Sierra, R. G.4, Author
Svenda, M.6, Author
van der Schot, G.6, Author
Vartanyants, I. A.14, 15, Author
Williams, G. J.16, Author
Paulraj, L. X.17, 18, Author              Aquila, A.4, AuthorZwart, P. H.3, 12, AuthorMancuso, A. P.1, Author more..
Affiliations:
1European XFEL GmbH, ou_persistent22              
2Mathematics Department, Lawrence Berkeley National Laboratory, ou_persistent22              
3Center for Advanced Mathematics for Energy Research Applications, ou_persistent22              
4Linac Coherent Light Source, SLAC National Accelerator Laboratory, ou_persistent22              
5Australian Research Council Centre of Excellence in Advanced Molecular Imaging, La Trobe Institute for Molecular Science, La Trobe University, ou_persistent22              
6Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, ou_persistent22              
7Biosciences Division, SLAC National Accelerator Laboratory, ou_persistent22              
8Stanford PULSE Institute, SLAC National Accelerator Laboratory, ou_persistent22              
9Biodesign Center for Applied Structural Discovery and School of Molecular Sciences Arizona State University, ou_persistent22              
10NERSC, Lawrence Berkeley National Laboratory, ou_persistent22              
11Division of Scientific Computing, Science for Life Laboratory, Department of Information Technology, Uppsala University, ou_persistent22              
12Molecular Biophysics and Integrated Bioimaging, Lawrence Berkeley National Laboratory, ou_persistent22              
13Biomedical and X-Ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology, ou_persistent22              
14Deutsches Elektronen-Synchrotron DESY, ou_persistent22              
15National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), ou_persistent22              
16NSLS-II, Brookhaven National Laboratory, ou_persistent22              
17International Max Planck Research School for Ultrafast Imaging & Structural Dynamics (IMPRS-UFAST), Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_2266714              
18Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, ou_persistent22              

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 Abstract: We use extremely bright and ultrashort pulses from an x-ray free-electron laser (XFEL) to measure correlations in x rays scattered from individual bioparticles. This allows us to go beyond the traditional crystallography and single-particle imaging approaches for structure investigations. We employ angular correlations to recover the three-dimensional (3D) structure of nanoscale viruses from x-ray diffraction data measured at the Linac Coherent Light Source. Correlations provide us with a comprehensive structural fingerprint of a 3D virus, which we use both for model-based and ab initio structure recovery. The analyses reveal a clear indication that the structure of the viruses deviates from the expected perfect icosahedral symmetry. Our results anticipate exciting opportunities for XFEL studies of the structure and dynamics of nanoscale objects by means of angular correlations.

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Language(s): eng - English
 Dates: 2017-05-222017-10-122017-10-12
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1103/PhysRevLett.119.158102
 Degree: -

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Project name : Portions of this research were carried out at the LCLS at the SLAC National Accelerator Laboratory. The LCLS is sup- ported by the U.S. Department of Energy (DOE), Office of Science, Office of Basic Energy Sciences (OBES), under Contract No. DE-AC02-76SF00515. This work was sup- ported by the European Research Council, “ Frontiers in Attosecond X-ray Science: Imaging and Spectroscopy (AXSIS), ” ERC-2013-SyG 609920. This work was also supported by the Swedish Research Council, the Knut and Alice Wallenberg Foundation, the European Research Council, the Röntgen Ångström Cluster, the Swedish Foundation for International Cooperation in Research and Higher Education (STINT), and the Swedish Foundation for Strategic Research. This work was supported by the OBES through the AMOS program within the CSGB and by the DOE through the SLAC Laboratory Directed Research and Development Program. It was also supported by the joint Stanford ChEM-H and SLAC National Accelerator Laboratory seed grant program. Part of this work was supported by the NSF Science and Technology Centers Grant No. NSF-1231306 ( “ Biology with X-ray Lasers, ” BioXFEL), NIH Grant No. R01GM095583. This work was supported by the Australian Research Council Centre of Excellence in Advanced Molecular Imaging (CE140100011) [76] . Portions of this research were carried out at Brookhaven National Laboratory, operated under Contract No. DE-SC0012704 from the U.S. Department of Energy (DOE) Office of Science. This research was supported in part by the Center for Advanced Mathematics for Energy Research Applications, which is funded by the Advanced Scientific Computing Research and the Basic Energy Sciences programs, which are supported by the Office of Science of the U.S. Department of Energy under Contract No. DEAC02-05CH11231. This research used resources of the National Energy Research Scientific Computing Center, a DOE Office of Science User Facility supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. Further support originated from the National Institute of General Medical Sciences of the National Institutes of Health under Grant No. R01GM109019. The content of this article is solely the responsibility of the authors and does not necessarily re- present the official views of the National Institutes of Health.
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Title: Physical Review Letters
  Abbreviation : Phys. Rev. Lett.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Woodbury, N.Y. : American Physical Society
Pages: - Volume / Issue: 119 (15) Sequence Number: 158102 Start / End Page: - Identifier: ISSN: 0031-9007
CoNE: /journals/resource/954925433406_1