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  Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals

Awel, S., Kirian, R. A., Wiedorn, M. O., Beyerlein, K. R., Roth, N., Horke, D. A., et al. (2018). Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals. Journal of Applied Crystallography, 51, 133-139. doi:10.1107/S1600576717018131.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-D60C-1 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-D60D-0
Genre: Journal Article

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te5021.pdf (Publisher version), 642KB
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2018
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https://dx.doi.org/10.1107/S1600576717018131 (Publisher version)
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 Creators:
Awel, S.1, 2, Author
Kirian, R. A.3, Author
Wiedorn, M. O.1, 4, Author
Beyerlein, K. R.1, Author
Roth, N.1, Author
Horke, D. A.1, 2, Author
Oberthür, D.1, Author
Knoska, J.1, 4, Author
Mariani, V.1, Author
Morgan, A.1, Author
Adriano, L.5, Author
Tolstikova, A.1, 4, Author
Paulraj, L. X.1, 6, Author              
Yefanov, O.1, Author
Aquila, A.7, Author
Barty, A.1, Author
Roy-Chowdhury, S.3, Author
Hunter, M. S.7, Author
James, D.3, Author
Robinson, J. S.7, Author
Weierstall, U.3, AuthorRode, A. V.8, AuthorBajt, S.5, AuthorKüpper, J.1, 2, 4, AuthorChapman, H. N.1, 2, 4, Author more..
Affiliations:
1Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, ou_persistent22              
2The Hamburg Center for Ultrafast Imaging, Universität Hamburg, ou_persistent22              
3Arizona State University, Tempe, ou_persistent22              
4Department of Physics, Universität Hamburg, ou_persistent22              
5Photon Science, Deutsches Elektronen-Synchrotron DESY, ou_persistent22              
6International Max Planck Research School for Ultrafast Imaging & Structural Dynamics (IMPRS-UFAST), Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_2266714              
7Linac Coherent Light Source (LCLS), SLAC National Accelerator Laborator, ou_persistent22              
8Laser Physics Centre, Research School of Physics and Engineering, Australian National University, ou_persistent22              

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Free keywords: X-ray diffraction, aerosols, nanocrystals
 Abstract: High-resolution Bragg diffraction from aerosolized single granulovirus nanocrystals using an X-ray free-electron laser is demonstrated. The outer dimensions of the in-vacuum aerosol injector components are identical to conventional liquid-microjet nozzles used in serial diffraction experiments, which allows the injector to be utilized with standard mountings. As compared with liquid-jet injection, the X-ray scattering background is reduced by several orders of magnitude by the use of helium carrier gas rather than liquid. Such reduction is required for diffraction measurements of small macromolecular nanocrystals and single particles. High particle speeds are achieved, making the approach suitable for use at upcoming high-repetition-rate facilities.

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Language(s): eng - English
 Dates: 2017-01-242017-12-192018-022018-02
 Publication Status: Published in print
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1107/S1600576717018131
 Degree: -

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Project name : We thank the LCLS staff for accommodating laser-light-scat- tering imaging in the CXI instrument during our experiments. Portions of this research were carried out at the LCLS at the SLAC National Accelerator Laboratory. This LCLS beamtime was part of the Protein Crystal Screening (PCS) program. LCLS is an Office of Science User Facility operated for the US Department of Energy, Office of Science, by Stanford University.
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Funding organization : -

Source 1

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Title: Journal of Applied Crystallography
  Abbreviation : J. Appl. Cryst.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Oxford, England : Blackwell Publishing on behalf of the International Union of Crystallography
Pages: - Volume / Issue: 51 Sequence Number: - Start / End Page: 133 - 139 Identifier: ISSN: 0021-8898
CoNE: /journals/resource/954925410812