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  A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins.

Lukinavicius, G., Umezawa, K., Olivier, N., Honigmann, A., Yang, G., Plass, T., et al. (2013). A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins. Nature Chemistry, 5(2), 132-139. doi:10.1038/nchem.1546.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-DAD6-8 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-DE97-B
Genre: Journal Article

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Lukinavicius, G.1, Author              
Umezawa, K., Author
Olivier, N., Author
Honigmann, A.2, Author              
Yang, G., Author
Plass, T., Author
Müller, V.2, Author
Reymond, L., Author
Correa Jr., I. R. , Author
Luo, Z. G., Author
Schultz, C., Author
Lemke, E. A., Author
Heppenstall, P., Author
Eggeling, C.2, Author              
Manley, S., Author
Johnsson, K., Author
Affiliations:
1Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society, ou_2616691              
2Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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 Abstract: The ideal fluorescent probe for bioimaging is bright, absorbs at long wavelengths and can be implemented flexibly in living cells and in vivo. However, the design of synthetic fluorophores that combine all of these properties has proved to be extremely difficult. Here, we introduce a biocompatible near-infrared silicon–rhodamine probe that can be coupled specifically to proteins using different labelling techniques. Importantly, its high permeability and fluorogenic character permit the imaging of proteins in living cells and tissues, and its brightness and photostability make it ideally suited for live-cell super-resolution microscopy. The excellent spectroscopic properties of the probe combined with its ease of use in live-cell applications make it a powerful new tool for bioimaging.

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Language(s): eng - English
 Dates: 2013-01-06
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/nchem.1546
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Title: Nature Chemistry
Source Genre: Journal
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Pages: - Volume / Issue: 5 (2) Sequence Number: - Start / End Page: 132 - 139 Identifier: -