hide
Free keywords:
Proteomics, DIVE, PIRL, tissue ablation, tandem mass spectrometry
Abstract:
Introduction: Human skin is the largest organ of integumentary system that protect the body from e
xternal environment and prevent
excessive fluid loss [1]. Scientists are closer than ever to understanding the proteomes of skin [2]. However, the protein
compositions in each type of cell layers, especially in epidermis, are not accurately defined. For fu
rther characterization of protein
composition in layers of human skin we have undertaken a proteomic approach by using an innovative, “one
-
step” extraction
and homogenization method, called DIVE (desorption by impulsive excitation of intramolecular vibrati
onal states of water
molecules in the cell), induced by irradiating tissue with a pico
-
second infrared laser (PIRL) [3]. The aim of this study was to test if
with DIVE epidermis and dermis can be ablated separately.
