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  Comprehensive Proteomic Investigation of Ebf1 Heterozygosity in Pro-B Lymphocytes Ulilizing Data Independent Acquisition

Musa, Y. R., Boller, S., Puchalska, M., Grosschedl, R., & Mittler, G. (2018). Comprehensive Proteomic Investigation of Ebf1 Heterozygosity in Pro-B Lymphocytes Ulilizing Data Independent Acquisition. Journal of Proteome Research, 17, 76-85. doi:10.1021/acs.jproteome.7b00369.

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https://pubs.acs.org/doi/10.1021/acs.jproteome.7b00369 (Verlagsversion)
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Musa, Yarub R.1, Autor
Boller, Sören1, Autor
Puchalska, Monika1, Autor
Grosschedl, Rudolf1, Autor           
Mittler, Gerhard1, Autor           
Affiliations:
1Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, 79108 Freiburg, DE, ou_2243640              

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Schlagwörter: DDA, DIA, PRM, EBF1, pro-B lymphocyte, transcription factor, data dependent acquisition
 Zusammenfassung: Early B cell factor 1 (EBF1) is one of the key transcription factors required for orchestrating B-cell lineage development. Although studies have shown that Ebf1 haploinsufficiency is involved in the development of leukemia, no study has been conducted that characterizes the global effect of Ebf1 heterozygosity on the proteome of pro-B lymphocytes. Here, we employ both data independent acquisition (DIA) and shotgun data dependent acquisition (DDA) workflows for profiling proteins that are differently expressed between Ebf1+/+ and Ebf1+/- cells. Both DDA and DIA were able to reveal the downregulation of the EBF1 transcription factor in Ebf1+/- pro-B lymphocytes. Further examination of differentially expressed proteins by DIA revealed that, similar to EBF1, the expression of other B-cell lineage regulators, such as TCF3 and Pax5, is also downregulated in Ebf1 heterozygous cells. Functional DIA analysis of differentially expressed proteins showed that EBF1 heterozygosity resulted in the deregulation of at least eight transcription factors involved in lymphopoiesis and the deregulation of key proteins playing crucial roles in survival, development, and differentiation of pro-B lymphocytes.

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Sprache(n): eng - English
 Datum: 2018
 Publikationsstatus: Erschienen
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 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1021/acs.jproteome.7b00369
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Titel: Journal of Proteome Research
  Andere : J. Proteome Res.
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Washington, D.C. : American Chemical Society
Seiten: - Band / Heft: 17 Artikelnummer: - Start- / Endseite: 76 - 85 Identifikator: ISSN: 1535-3893
CoNE: https://pure.mpg.de/cone/journals/resource/111019664290000