English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Comprehensive Proteomic Investigation of Ebf1 Heterozygosity in Pro-B Lymphocytes Ulilizing Data Independent Acquisition

Musa, Y. R., Boller, S., Puchalska, M., Grosschedl, R., & Mittler, G. (2018). Comprehensive Proteomic Investigation of Ebf1 Heterozygosity in Pro-B Lymphocytes Ulilizing Data Independent Acquisition. Journal of Proteome Research, 17, 76-85. doi:10.1021/acs.jproteome.7b00369.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0002-4857-C Version Permalink: http://hdl.handle.net/21.11116/0000-0007-AC96-E
Genre: Journal Article

Files

show Files
hide Files
:
Musa et al..pdf (Publisher version), 2MB
Name:
Musa et al..pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Musa, Yarub R.1, Author
Boller, Sören1, Author
Puchalska, Monika1, Author
Grosschedl, Rudolf1, Author              
Mittler, Gerhard1, Author              
Affiliations:
1Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, 79108 Freiburg, DE, ou_2243640              

Content

show
hide
Free keywords: DDA, DIA, PRM, EBF1, pro-B lymphocyte, transcription factor, data dependent acquisition
 Abstract: Early B cell factor 1 (EBF1) is one of the key transcription factors required for orchestrating B-cell lineage development. Although studies have shown that Ebf1 haploinsufficiency is involved in the development of leukemia, no study has been conducted that characterizes the global effect of Ebf1 heterozygosity on the proteome of pro-B lymphocytes. Here, we employ both data independent acquisition (DIA) and shotgun data dependent acquisition (DDA) workflows for profiling proteins that are differently expressed between Ebf1+/+ and Ebf1+/- cells. Both DDA and DIA were able to reveal the downregulation of the EBF1 transcription factor in Ebf1+/- pro-B lymphocytes. Further examination of differentially expressed proteins by DIA revealed that, similar to EBF1, the expression of other B-cell lineage regulators, such as TCF3 and Pax5, is also downregulated in Ebf1 heterozygous cells. Functional DIA analysis of differentially expressed proteins showed that EBF1 heterozygosity resulted in the deregulation of at least eight transcription factors involved in lymphopoiesis and the deregulation of key proteins playing crucial roles in survival, development, and differentiation of pro-B lymphocytes.

Details

show
hide
Language(s): eng - English
 Dates: 2018
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1021/acs.jproteome.7b00369
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Proteome Research
  Other : J. Proteome Res.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Washington, D.C. : American Chemical Society
Pages: - Volume / Issue: 17 Sequence Number: - Start / End Page: 76 - 85 Identifier: ISSN: 1535-3893
CoNE: https://pure.mpg.de/cone/journals/resource/111019664290000