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  Targeted photoswitchable probe for nanoscopy of biological structures.

Dellagiacoma , C., Lukinavicius, G., Boccio, N., Banala, S., Geissbühler, S., Märki, I., et al. (2010). Targeted photoswitchable probe for nanoscopy of biological structures. ChemBioChem, 11(10), 1361-1363. doi:10.1002/cbic.201000189.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-DED7-3 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-DEDB-F
Genre: Journal Article

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2628651.pdf (Publisher version), 467KB
 
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Dellagiacoma , C., Author
Lukinavicius, G.1, Author              
Boccio, N., Author
Banala, S., Author
Geissbühler, S., Author
Märki, I., Author
Johnsson, K., Author
Lasser, T., Author
Affiliations:
1Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society, ou_2616691              

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 Abstract: SNAP‐tag: We introduce a photoswitchable O6‐benzylguanine derivative and demonstrate its use for super‐resolution microscopy of SNAP‐tagged proteins based on single fluorophore localization. Stochastic Optical Reconstruction Microscopy (STORM) reveals SNAP‐tagged microtubule structures with ∼25 nm resolution. The described probe in combination with the versatile SNAP‐tag labeling opens new possibilities for imaging biological structures at the nanoscale.

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Language(s): eng - English
 Dates: 2010-06-202010-07-05
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1002/cbic.201000189
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Title: ChemBioChem
Source Genre: Journal
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Pages: - Volume / Issue: 11 (10) Sequence Number: - Start / End Page: 1361 - 1363 Identifier: -