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  Targeted labeling of DNA by methyltransferase-directed transfer of activated groups (mTAG).

Lukinavicius, G., Lapienė, V., Staševskij, Z., Dalhoff, C., Weinhold, E., & Klimašauskas, S. (2007). Targeted labeling of DNA by methyltransferase-directed transfer of activated groups (mTAG). Journal of the American Chemical Society, 129(10), 2758-2759. doi:10.1021/ja0691876.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-DFEE-9 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-DFF2-3
Genre: Journal Article

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2629783.pdf (Publisher version), 78KB
 
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Lukinavicius, G.1, Author              
Lapienė, V., Author
Staševskij, Z., Author
Dalhoff, C., Author
Weinhold, E., Author
Klimašauskas, S., Author
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1Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society, ou_2616691              

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 Abstract: Methyltransferases catalyze highly specific transfers of methyl groups from the ubiquitous cofactor S-adenosyl-l-methionine (AdoMet) to various biopolymers like DNA, RNA, and proteins. Here we describe the first synthetic analogue of AdoMet with an activated side chain carrying a primary amino group that permits efficient methyltransferase-directed functionalization of DNA and subsequent amine-specific chemoligations with various reporter groups. The demonstrated two-step sequence-specific labeling of natural DNA offers a facile way to query the methylation status of the target sites and envisions numerous applications in functional studies and medical diagnostics.

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Language(s): eng - English
 Dates: 2007-02-202007
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1021/ja0691876
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Title: Journal of the American Chemical Society
Source Genre: Journal
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Pages: - Volume / Issue: 129 (10) Sequence Number: - Start / End Page: 2758 - 2759 Identifier: -