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  A microfluidic biochip for locally confined stimulation of cells within an epithelial monolayer

Thuenauer, R., Nicklaus, S., Frensch, M., Troendle, K., Madl, J., & Römer, W. (2018). A microfluidic biochip for locally confined stimulation of cells within an epithelial monolayer. RSC Advances, 8, 7839-7846. doi:10.1039/c7ra11943g.

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Datensatz-Permalink: https://hdl.handle.net/21.11116/0000-0002-7165-D Versions-Permalink: https://hdl.handle.net/21.11116/0000-0002-7166-C
Genre: Zeitschriftenartikel

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 Urheber:
Thuenauer, Roland1, Autor
Nicklaus, Simon1, Autor
Frensch, Marco2, Autor
Troendle, Kevin1, Autor
Madl, Josef1, Autor
Römer, Winfried1, Autor
Affiliations:
1External Organizations, ou_persistent22              
2Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, 79108 Freiburg, DE, ou_2243640              

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 Zusammenfassung: A key factor determining the fate of individual cells within an epithelium is the unique microenvironment that surrounds each cell. It regulates location-dependent differentiation into specific cellular sub-types, but, on the other hand, a disturbed microenvironment can promote malignant transformation of epithelial cells leading to cancer formation. Here, we present a tool based on a microfluidic biochip that enables novel research approaches by providing a means to control the basolateral micro-environment of a confined number of neighbouring cells within an epithelial monolayer. Through isolated single pores in a thin membrane carrying the epithelial cell layer only cells above the pores are stimu-lated by solutes. The very thin design of the biochip (<75 μm) enabled us to apply a high-resolution inverted confocal fluore-scence microscope to show by live cell imaging that such a manipulation of the microenvironment remained locally restricted to cells located above the pores. In addition, the biochip allows access for the force probe of an atomic force microscope (AFM) from the apical side to determine the topography and mechanical properties of individual cells, which we demonstrated by combined AFM and fluorescence microscopy imaging experiments. Taken together, the presented microfluidic biochip is a powerful tool that will enable studying the initial steps of malignant transformation of epithelial cells by directly manipulating their micro-environment and by real-time monitoring of affected cells with fluorescence microscopy and AFM.

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Sprache(n): eng - English
 Datum: 2018
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1039/c7ra11943g
 Art des Abschluß: -

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Titel: RSC Advances
  Kurztitel : RSC Adv.
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Cambridge, UK : Royal Society of Chemistry
Seiten: - Band / Heft: 8 Artikelnummer: - Start- / Endseite: 7839 - 7846 Identifikator: ISSN: 2046-2069
CoNE: https://pure.mpg.de/cone/journals/resource/2046-2069