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  Fluorescent labeling of SNAP-tagged proteins in cells.

Lukinavicius, G., Reymond, L., & Johnsson, K. (2015). Fluorescent labeling of SNAP-tagged proteins in cells. In A. Gautier, & M. J. Hinner (Eds.), Site-Specific Protein Labeling (pp. 107-118). New York, N.Y.: Springer Nature. doi:10.1007/978-1-4939-2272-7_7.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-E423-6 Version Permalink: http://hdl.handle.net/21.11116/0000-0002-0DF7-A
Genre: Book Chapter

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2630240.pdf (Publisher version), 844KB
 
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 Creators:
Lukinavicius, G.1, Author              
Reymond, L., Author
Johnsson, K., Author
Affiliations:
1Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society, ou_2616691              

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 Abstract: One of the most prominent self-labeling tags is SNAP-tag. It is an in vitro evolution product of the human DNA repair protein O 6-alkylguanine-DNA alkyltransferase (hAGT) that reacts specifically with benzylguanine (BG) and benzylchloropyrimidine (CP) derivatives, leading to covalent labeling of SNAP-tag with a synthetic probe (Gronemeyer et al., Protein Eng Des Sel 19:309–316, 2006; Curr Opin Biotechnol 16:453–458, 2005; Keppler et al., Nat Biotechnol 21:86–89, 2003; Proc Natl Acad Sci U S A 101:9955–9959, 2004). SNAP-tag is well suited for the analysis and quantification of fused target protein using fluorescence microscopy techniques. It provides a simple, robust, and versatile approach to the imaging of fusion proteins under a wide range of experimental conditions.

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Language(s): eng - English
 Dates: 2014-12-082015
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1007/978-1-4939-2272-7_7
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Title: Site-Specific Protein Labeling
Source Genre: Book
 Creator(s):
Gautier, A., Editor
Hinner, M. J., Editor
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Publ. Info: New York, N.Y. : Springer Nature
Pages: xii, 267 Volume / Issue: - Sequence Number: - Start / End Page: 107 - 118 Identifier: ISBN: 978-1-4939-2271-0

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Title: Methods in Molecular Biology
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Pages: - Volume / Issue: 1266 Sequence Number: - Start / End Page: - Identifier: -