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  The regulator of G‐protein signaling Thn1 links pheromone response to volatile production in Schizophyllum commune

Wirth, S., Kunert, M., Ahrens, L., Krause, K., Broska, S., Paetz, C., et al. (2018). The regulator of G‐protein signaling Thn1 links pheromone response to volatile production in Schizophyllum commune. Environmental Microbiology, 20(10), 3684-3699. doi:10.1111/1462-2920.14369.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0001-E6DC-4 Version Permalink: https://hdl.handle.net/21.11116/0000-0009-3116-7
Genre: Journal Article

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 Creators:
Wirth, Sophia, Author
Kunert, Maritta1, Author           
Ahrens, Lisa‐Marija, Author
Krause, Katrin, Author
Broska, Selina, Author
Paetz, Christian2, Author           
Kniemeyer, Olaf, Author
Jung, Elke‐Martina, Author
Boland, Wilhelm1, Author           
Kothe, Erika, Author
Affiliations:
1Department of Bioorganic Chemistry, Prof. Dr. W. Boland, MPI for Chemical Ecology, Max Planck Society, ou_24028              
2Research Group Biosynthesis / NMR, MPI for Chemical Ecology, Max Planck Society, ou_421898              

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 Abstract: The regulator of G-protein signaling, Thn1, is involved in sexual development through pheromone signaling in the mushroom forming basidiomycete Schizophyllum commune affecting hyphal morphology and mating interactions. Thn1 plays a key role in coordinating sesquiterpene production, pheromone response and sexual development. The gene thn1 is transcriptionally regulated in response to mating with a role in clamp cell development and hydrophobin gene transcription. Further, it negatively regulates cAMP signaling and secondary metabolism. Disruption of thn1 affects dikaryotization by reducing clamp fusion and development with predominant non-fused pseudoclamps. Enhanced protein kinase A (PKA) activities in Δthn1 strains indicate that Thn1 regulates pheromone signaling by de-activating G-protein α subunits, which control cAMP-dependent PKA. The repressed formation of aerial hyphae could be linked to a reduced metabolic activity and to a transcriptional down-regulation of hyd6 and sc3 hydrophobin genes. Thn1 was also shown to be necessary for the biosynthesis of sesquiterpenes and an altered spectrum of sesquiterpenes in Δthn1 is linked to transcriptional up-regulation of biosynthesis genes. Proteome analysis indicated changes in cytoskeletal structure affecting actin localization, linking the major regulator Thn1 to growth and development of S. commune. The results support a role for Thn1 in G-protein signaling connecting development and secondary metabolism.

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 Dates: 2018-07-302018-07-30
 Publication Status: Published online
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 Identifiers: Other: BOL703
DOI: 10.1111/1462-2920.14369
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Title: Environmental Microbiology
  Other : Environmental Microbiology and Environmental Microbiology Reports
Source Genre: Journal
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Publ. Info: Oxford, England : Blackwell Science
Pages: - Volume / Issue: 20 (10) Sequence Number: - Start / End Page: 3684 - 3699 Identifier: ISSN: 1462-2912
CoNE: https://pure.mpg.de/cone/journals/resource/959328105031