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  Expression, purification and preliminary crystallographic characterization of a novel segment from the neurofibromatosis type 1 protein

Bonneau, F., D Angelo, I., Welti, S., Stier, G., Ylänne, J., & Scheffzek, K. (2004). Expression, purification and preliminary crystallographic characterization of a novel segment from the neurofibromatosis type 1 protein. Acta Crystallographica Section D-Biological Crystallography, 60(2), 2364-2367. doi:10.1107/S0907444904026861.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0001-E809-0 Version Permalink: https://hdl.handle.net/21.11116/0000-0001-E80A-F
Genre: Journal Article

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ActaCrystD_60_2004_2364.pdf (Any fulltext), 243KB
 
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http://journals.iucr.org/d/issues/2004/12/02/bw5061/bw5061.pdf (Any fulltext)
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 Creators:
Bonneau, F., Author
D Angelo, I., Author
Welti, S., Author
Stier, Gunter1, Author           
Ylänne, J., Author
Scheffzek, Klaus2, Author           
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              
2Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              

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Free keywords: tumour suppressor; cancer; RasGAP; CRAL−TRIO
 Abstract: Neurofibromin (MW 320 kDa) is the protein responsible for the pathogenesis of neurofibromatosis type 1 (NF1), one of the most common genetic diseases worldwide. The neurofibromin GAP-related domain (GRD, MW 38 kDa) possess a Ras-specific GTPase-activating protein property, which is at present its only clear biochemical function. This article describes the study of the bacterial production and preliminary X-ray crystallographic analysis of a neurofibromin fragment located at the C-terminal end of the GRD, which contains a region reported to be homologous to the yeast Sec14p lipid exchange protein. Of the three crystal variants obtained, a tetragonal form diffracted to a resolution of at least 2.3 A.

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Language(s): eng - English
 Dates: 2004-08-042004-10-222004-11-262004-12-01
 Publication Status: Issued
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1107/S0907444904026861
URI: http://www.ncbi.nlm.nih.gov/pubmed/15583390
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Title: Acta Crystallographica Section D-Biological Crystallography
Source Genre: Journal
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Publ. Info: [Copenhagen, Denmark : Published for the International Union of Crystallography by Munksgaard]
Pages: - Volume / Issue: 60 (2) Sequence Number: - Start / End Page: 2364 - 2367 Identifier: ISSN: 0907-4449
CoNE: https://pure.mpg.de/cone/journals/resource/954925562619_1