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  Small synthetic molecule-stabilized RNA pseudoknot as an activator for -1 ribosomal frameshifting.

Matsumoto, S., Calsikan, N., Rodnina, M. V., Murata, A., & Nakatani, K. (2018). Small synthetic molecule-stabilized RNA pseudoknot as an activator for -1 ribosomal frameshifting. Nucleic Acids Research, 46(16), 8079-8089. doi:10.1093/nar/gky689.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-EA7D-C Version Permalink: http://hdl.handle.net/21.11116/0000-0003-A86C-7
Genre: Journal Article

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 Creators:
Matsumoto, S., Author
Calsikan, N., Author
Rodnina, M. V.1, Author              
Murata, A., Author
Nakatani, K., Author
Affiliations:
1Department of Physical Biochemistry, MPI for Biophysical Chemistry, Max Planck Society, ou_578598              

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 Abstract: Programmed -1 ribosomal frameshifting (-1PRF) is a recoding mechanism to make alternative proteins from a single mRNA transcript. -1PRF is stimulated by cis-acting signals in mRNA, a seven-nucleotide slippery sequence and a downstream secondary structure element, which is often a pseudoknot. In this study we engineered the frameshifting pseudoknot from the mouse mammary tumor virus to respond to a rationally designed small molecule naphthyridine carbamate tetramer (NCTn). We demonstrate that NCTn can stabilize the pseudoknot structure in mRNA and activate -1PRF both in vitro and in human cells. The results illustrate how NCTn-inducible -1PRF may serve as an important component of the synthetic biology toolbox for the precise control of gene expression using small synthetic molecules.

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Language(s): eng - English
 Dates: 2018-08-022018-09-19
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1093/nar/gky689
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Title: Nucleic Acids Research
Source Genre: Journal
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Pages: - Volume / Issue: 46 (16) Sequence Number: - Start / End Page: 8079 - 8089 Identifier: -