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  Genetically encoded FRET probe for PKC activity based on pleckstrin

Schleifenbaum, A., Stier, G., Gasch, A., Sattler, M., & Schultz, C. (2004). Genetically encoded FRET probe for PKC activity based on pleckstrin. Journal of the American Chemical Society, 126(38), 11786-11787. doi:10.1021/ja0460155.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0001-EC06-F Version Permalink: https://hdl.handle.net/21.11116/0000-0001-EC07-E
Genre: Journal Article

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JACS_126_2004_11786_Suppl.pdf (Supplementary material), 501KB
 
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 Creators:
Schleifenbaum, A., Author
Stier, Gunter1, Author           
Gasch, A., Author
Sattler, Michael, Author
Schultz, C., Author
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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 Abstract: We developed a probe for investigating protein kinase C (PKC) activity in living cells. The probe is based on a fragment of pleckstrin enclosed by two FRET-capable fluorophores. PKC activity modulation was reliably followed by FRET change in vitro and in vivo. The probe responds quickly to PKC activation by phorbol ester. FRET changes were reversible when PKC inhibitors were administered. Stimulation of cellular signaling pathways using histamine or bradykinin triggered PKC in a physiologically relevant way.

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Language(s): eng - English
 Dates: 2004-07-052004-09-022004-09-022004-09-29
 Publication Status: Issued
 Pages: 2
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1021/ja0460155
URI: https://www.ncbi.nlm.nih.gov/pubmed/15382901
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Title: Journal of the American Chemical Society
  Other : J. Am. Chem. Soc.
  Abbreviation : JACS
Source Genre: Journal
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Publ. Info: American Chemical Society
Pages: - Volume / Issue: 126 (38) Sequence Number: - Start / End Page: 11786 - 11787 Identifier: ISSN: 0002-7863
CoNE: https://pure.mpg.de/cone/journals/resource/954925376870