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  The solubility and stability of recombinant proteins are increased by their fusion to NusA

De Marco, V., Stier, G., Blandin, S., & de Marco, A. (2004). The solubility and stability of recombinant proteins are increased by their fusion to NusA. Biochemical and Biophysical Research Communications, 322(3), 766-771. doi:10.1016/j.bbrc.2004.07.189.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0001-EC0B-A Version Permalink: https://hdl.handle.net/21.11116/0000-0001-EC0C-9
Genre: Journal Article

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BiochemBiophysResComm_322_2004_766.pdf (Any fulltext), 672KB
 
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 Creators:
De Marco, Valeria, Author
Stier, Gunter1, Author           
Blandin, S., Author
de Marco, Ario, Author
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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 Abstract: The new bacterial vector pETM60 enables the expression of His-tagged recombinant proteins fused to the C-terminus of NusA through a TEV protease recognition sequence. Three sequences coding for two protein domains (Xklp3A and Tep3Ag) and one membrane-bound viral protein (E8R) could not be expressed in a soluble form in bacteria. Their GST-fusions were mostly soluble but quickly degraded during purification. The same sequences cloned in pETM60 were efficiently purified by metal affinity and recovered soluble after the removal of the fusion partner. The NusA-fused constructs enabled to yield 13-20mg of fusion protein per litre of culture and 2.5-5mg of pure protein per litre of culture. Structural analysis indicated that the purified proteins were monodispersed and correctly folded. NusA has been used to raise antibodies that have been successfully used for Western blot and immunoprecipitation of NusA fusion proteins.

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Language(s): eng - English
 Dates: 2004-07-262004-08-202004-09-24
 Publication Status: Issued
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.bbrc.2004.07.189
URI: http://www.ncbi.nlm.nih.gov/pubmed/15336530
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Title: Biochemical and Biophysical Research Communications
  Other : Biochem. Biophys. Res. Commun.
Source Genre: Journal
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Publ. Info: Orlando, Fla. : Academic Press
Pages: - Volume / Issue: 322 (3) Sequence Number: - Start / End Page: 766 - 771 Identifier: ISSN: 0006-291X
CoNE: https://pure.mpg.de/cone/journals/resource/954922652205_1