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  Single-Nanoparticle Cell Barcoding by Tunable FRET from Lanthanides to Quantum Dots

Chen, C., Ao, L., Wu, Y.-T., Cifliku, V., Cardoso Dos Santos, M., Bourrier, E., et al. (2018). Single-Nanoparticle Cell Barcoding by Tunable FRET from Lanthanides to Quantum Dots. Angewandte Chemie International Edition, 57(41), 13686-13690. doi:10.1002/anie.201807585.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-EDAB-4 Version Permalink: http://hdl.handle.net/21.11116/0000-0007-19D3-F
Genre: Journal Article
Other : Einzelnanopartikel‐Strichkodierung von Zellen mittels durchstimmbarem FRET von Lanthanoiden auf Quantenpunkte

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 Creators:
Chen, Chi, Author
Ao, Lijiao, Author
Wu, Yu-Tang, Author
Cifliku, Vjona, Author
Cardoso Dos Santos, Marcelina, Author
Bourrier, Emmanuel, Author
Delbianco, Martina1, Author              
Parker, David, Author
Zwier, Jurriaan, Author
Huang, Liang, Author
Hildebrandt, Niko, Author
Affiliations:
1Martina Delbianco, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_2559692              

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Free keywords: FRET, Lanthanides, quantum dots, Lifetime, imaging
 Abstract: Fluorescence barcoding based on nanoparticles provides many advantages for multiparameter imaging. However, creating different concentration-independent codes without mixing various nanoparticles and by using single wavelength excitation and emission for multiplexed cellular imaging is extremely challenging. Here, we report the development of quantum dots (QDs) with two different SiO2 shell thicknesses (6 and 12 nm) and coated with two different lanthanide complexes (Tb and Eu). FRET from Tb or Eu donors to the QD acceptors resulted in four distinct photoluminescence (PL) decays, which were encoded by simple time-gated (TG) PL intensity detection in three individual temporal detection windows. The well-defined single-nanoparticle codes were used for live cell imaging and a one-measurement distinction of four different cells in a single field of view. This single-color barcoding strategy opens new opportunities for multiplexed labeling and tracking of cells.
 Abstract: Fluoreszenzstrichkodierung mittels Nanopartikeln bietet viele Vorteile für die Multiparameter‐Mikroskopie. Es ist jedoch extrem schwierig, verschiedene konzentrationsunabhängige Codes zu erstellen, ohne verschiedene Nanopartikel zu mischen und Anregung und Emission einer einzelnen Wellenlänge für die zelluläre Multiplex‐Bildgebung zu verwenden. Hier berichten wir über die Entwicklung von Quantenpunkten (quantum dots: QDs) mit zwei unterschiedlichen SiO2‐Schalenstärken (6 und 12 nm), die mit zwei verschiedenen Lanthanoidkomplexen (Tb und Eu) beschichtet sind. FRET von Tb‐ oder Eu‐Donoren auf QD‐Akzeptoren führte zu vier unterschiedlichen Photolumineszenz(PL)‐Abklingkurven, die durch einfache zeitgetaktete (time‐gated: TG) PL‐Intensitätsdetektion in drei individuellen temporalen Detektionsfenstern kodiert wurden. Die wohldefinierten Einzelnanopartikel‐Codes wurden für die Lebendzellen‐Mikroskopie und die Unterscheidung von vier verschiedenen Zellen in einem einzigen Sichtfeld mittels einer Messung verwendet. Diese Einzelfarben‐Strichkodierungsstrategie eröffnet neue Möglichkeiten für die Multiplexmarkierung und Verfolgung von Zellen.

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Language(s): eng - English, deu - German
 Dates: 2018-08-072018
 Publication Status: Published in print
 Pages: -
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Source 1

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Title: Angewandte Chemie International Edition
  Other : Angewandte Chemie, International Edition
  Other : Angew. Chem. Int. Ed.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: 57 (41) Sequence Number: - Start / End Page: 13686 - 13690 Identifier: ISSN: 1433-7851

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Title: Angewandte Chemie
  Abbreviation : Angew. Chem.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: 130 (41) Sequence Number: - Start / End Page: 13876 - 13881 Identifier: ISSN: 0044-8249