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  Combining adhesive nanostructured surfaces and costimulatory signals to increase T cell activation

Guasch, J., Hoffmann, M., Diemer, J., Riahinezhad, H., Neubauer, S., Kessler, H., et al. (2018). Combining adhesive nanostructured surfaces and costimulatory signals to increase T cell activation. Nano Letters, 18(9), 5899-5904. doi:10.1021/acs.nanolett.8b02588.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0001-ECFC-A Version Permalink: https://hdl.handle.net/21.11116/0000-0002-6C3F-0
Genre: Journal Article

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 Creators:
Guasch, Judith, Author
Hoffmann, Marco1, 2, Author           
Diemer, Jennifer1, 2, Author           
Riahinezhad, Hossein1, 2, Author           
Neubauer, Stefanie, Author
Kessler, Horst, Author
Spatz, Joachim P.1, 2, Author           
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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Free keywords: CD3/CD28; integrins; Nanostructures; PMA/ionomycin; T cells
 Abstract: Adoptive cell therapies are showing very promising results in the fight against cancer. However, these therapies are expensive and technically challenging in part due to the need of a large number of specific T cells, which must be activated and expanded in vitro. Here we describe a method to activate primary human T cells using a combination of nanostructured surfaces functionalized with the stimulating anti-CD3 antibody and the peptidic sequence arginine-glycine-aspartic acid, as well as costimulatory agents (anti-CD28 antibody and a cocktail of phorbol 12-myristate 13-acetate, ionomycin, and protein transport inhibitors). Thus, we propose a method that combines nanotechnology with cell biology procedures to efficiently produce T cells in the laboratory, challenging the current state-of-the-art expansion methodologies.

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Language(s): eng - English
 Dates: 2018-07-272018-06-262018-08-082018-08-082018-08-08
 Publication Status: Issued
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1021/acs.nanolett.8b02588
URI: https://www.ncbi.nlm.nih.gov/pubmed/30088769
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Title: Nano Letters
  Abbreviation : Nano Lett.
Source Genre: Journal
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Publ. Info: Washington, DC : American Chemical Society
Pages: - Volume / Issue: 18 (9) Sequence Number: - Start / End Page: 5899 - 5904 Identifier: ISSN: 1530-6984
CoNE: https://pure.mpg.de/cone/journals/resource/110978984570403