English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Extended darkness induces internal turnover of glucosinolates in Arabidopsis thaliana leaves

Brandt, S., Fachinger, S., Tohge, T., Fernie, A. R., Braun, H.-P., & Hildebrandt, T. M. (2018). Extended darkness induces internal turnover of glucosinolates in Arabidopsis thaliana leaves. PLoS One, 13(8), 1-15. doi:10.1371/journal.pone.0202153.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0001-F1CA-B Version Permalink: http://hdl.handle.net/21.11116/0000-0001-F1CB-A
Genre: Journal Article

Files

show Files

Locators

show
hide
Locator:
Link (Any fulltext)
Description:
-

Creators

show
hide
 Creators:
Brandt, Saskia1, Author
Fachinger, Sara1, Author
Tohge, T.2, Author              
Fernie, A. R.2, Author              
Braun, Hans-Peter1, Author
Hildebrandt, Tatjana M.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

Content

show
hide
Free keywords: -
 Abstract: Prolonged darkness leads to carbohydrate starvation, and as a consequence plants degrade proteins and lipids to oxidize amino acids and fatty acids as alternative substrates for mitochondrial ATP production. We investigated, whether the internal breakdown of glucosinolates, a major class of sulfur-containing secondary metabolites, might be an additional component of the carbohydrate starvation response in Arabidopsis thaliana (A. thaliana). The glucosinolate content of A. thaliana leaves was strongly reduced after seven days of darkness. We also detected a significant increase in the activity of myrosinase, the enzyme catalyzing the initial step in glucosinolate breakdown, coinciding with a strong induction of the main leaf myrosinase isoforms TGG1 and TGG2. In addition, nitrilase activity was increased suggesting a turnover via nitriles and carboxylic acids. Internal degradation of glucosinolates might also be involved in diurnal or developmental adaptations of the glucosinolate profile. We observed a diurnal rhythm for myrosinase activity in two-week-old plants. Furthermore, leaf myrosinase activity and protein abundance of TGG2 varied during plant development, whereas leaf protein abundance of TGG1 remained stable indicating regulation at the transcriptional as well as post-translational level.

Details

show
hide
Language(s): eng - English
 Dates: 2018-09
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1371/journal.pone.0202153
BibTex Citekey: 10.1371/journal.pone.0202153
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: PLoS One
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: San Francisco, CA : Public Library of Science
Pages: - Volume / Issue: 13 (8) Sequence Number: - Start / End Page: 1 - 15 Identifier: ISSN: 1932-6203
CoNE: /journals/resource/1000000000277850