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  Photothermal Control of Heat-Shock Protein Expression at the Single Cell Level

Robert, H. M. L., Savatier, J., Vial, S., Verghese, J., Wattellier, B., Rigneault, H., et al. (2018). Photothermal Control of Heat-Shock Protein Expression at the Single Cell Level. Small, 14(32): 1801910. doi:10.1002/smll.201801910.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-F7AA-9 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-F7AB-8
Genre: Journal Article

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 Creators:
Robert, Hadrien M. L.1, Author
Savatier, Julien1, Author
Vial, Stephanie1, Author
Verghese, Jacob2, Author              
Wattellier, Benoit1, Author
Rigneault, Herve1, Author
Monneret, Serge1, Author
Polleux, Julien3, Author              
Baffou, Guillaume1, Author
Affiliations:
1external, ou_persistent22              
2Hartl, Franz-Ulrich / Cellular Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565152              
3Fässler, Reinhard / Molecular Medicine, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565147              

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Free keywords: LIVING CELLS; INTRACELLULAR TEMPERATURE; FLUORESCENT THERMOSENSORS; STRESS; THERMOMETRY; THERMOGENESIS; NANOPARTICLES; GRANULES; NANORODS; FACTOR-1Chemistry; Science & Technology - Other Topics; Materials Science; Physics; cell imaging; gold nanoparticles; heat-shock; photothermal; plasmonics;
 Abstract: Laser heating of individual cells in culture recently led to seminal studies in cell poration, fusion, migration, or nanosurgery, although measuring the local temperature increase in such experiments remains a challenge. Here, the laser-induced dynamical control of the heat-shock response is demonstrated at the single cell level, enabled by the use of light-absorbing gold nanoparticles as nanosources of heat and a temperature mapping technique based on quadriwave lateral shearing interferometry (QLSI) measurements. As it is label-free, this approach does not suffer from artifacts inherent to previously reported fluorescence-based temperature-mapping techniques and enables the use of any standard fluorescent labels to monitor in parallel the cell's response.

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Language(s): eng - English
 Dates: 2018-08-09
 Publication Status: Published online
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: ISI: 000441134400020
DOI: 10.1002/smll.201801910
 Degree: -

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Title: Small
  Other : Small
Source Genre: Journal
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Publ. Info: Weinheim, Germany : Wiley
Pages: - Volume / Issue: 14 (32) Sequence Number: 1801910 Start / End Page: - Identifier: ISSN: 1613-6810
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000017440_1