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Free keywords:
LIVING CELLS; INTRACELLULAR TEMPERATURE; FLUORESCENT THERMOSENSORS;
STRESS; THERMOMETRY; THERMOGENESIS; NANOPARTICLES; GRANULES; NANORODS;
FACTOR-1Chemistry; Science & Technology - Other Topics; Materials Science;
Physics; cell imaging; gold nanoparticles; heat-shock; photothermal; plasmonics;
Abstract:
Laser heating of individual cells in culture recently led to seminal studies in cell poration, fusion, migration, or nanosurgery, although measuring the local temperature increase in such experiments remains a challenge. Here, the laser-induced dynamical control of the heat-shock response is demonstrated at the single cell level, enabled by the use of light-absorbing gold nanoparticles as nanosources of heat and a temperature mapping technique based on quadriwave lateral shearing interferometry (QLSI) measurements. As it is label-free, this approach does not suffer from artifacts inherent to previously reported fluorescence-based temperature-mapping techniques and enables the use of any standard fluorescent labels to monitor in parallel the cell's response.
