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Free keywords:
DNA-SEQUENCING DATA; COLORECTAL-CANCER; MASS-SPECTROMETER; RAS
MUTATIONS; CELL MOTILITY; GENE SET; GENOME; CARCINOMA; GENERATION;
PERIOSTINOncology; case study; clinical proteomics; epigenetics; mass spectrometry; urachal
carcinoma;
Abstract:
Recent advances in mass spectrometry (MS)-based technologies are now set to transform translational cancer proteomics from an idea to a practice. Here, we present a robust proteomic workflow for the analysis of clinically relevant human cancer tissues that allows quantitation of thousands of tumor proteins in several hours of measuring time and a total turnaround of a few days. We applied it to a chemorefractory metastatic case of the extremely rare urachal carcinoma. Quantitative comparison of lung metastases and surrounding tissue revealed several significantly upregulated proteins, among them lysine-specific histone demethylase 1 (LSD1/KDM1A). LSD1 is an epigenetic regulator and the target of active development efforts in oncology. Thus, clinical cancer proteomics can rapidly and efficiently identify actionable therapeutic options. While currently described for a single case study, we envision that it can be applied broadly to other patients in a similar condition.
