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  Distinct and evolutionary conserved structural features of the human nuclear exosome complex

Gerlach, P., Schuller, J. M., Bonneau, F., Basquin, J., Reichelt, P., Falk, S., et al. (2018). Distinct and evolutionary conserved structural features of the human nuclear exosome complex. eLife, 7: e38686. doi:10.7554/eLife.38686.

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 Creators:
Gerlach, Piotr1, Author              
Schuller, Jan M.1, Author              
Bonneau, Fabien1, Author              
Basquin, Jerome1, Author              
Reichelt, Peter1, Author              
Falk, Sebastian1, Author              
Conti, Elena1, Author              
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1Conti, Elena / Structural Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565144              

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Free keywords: CYTOPLASMIC MESSENGER-RNA; HUMAN PM-SCL; RIBOSOMAL-RNA; YEAST EXOSOME; CORE EXOSOME; CRYO-EM; PIGGYBAC TRANSPOSASE; DEGRADATION PATHWAY; LIN28-LET-7 PATHWAY; MULTIPLE-MYELOMALife Sciences & Biomedicine - Other Topics;
 Abstract: The nuclear RNA exosome complex mediates the processing of structured RNAs and the decay of aberrant non-coding RNAs, an important function particularly in human cells. Most mechanistic studies to date have focused on the yeast system. Here, we reconstituted and studied the properties of a recombinant 14-subunit human nuclear exosome complex. In biochemical assays, the human exosome embeds a longer RNA channel than its yeast counterpart. The 3.8 angstrom resolution cryo-EM structure of the core complex bound to a single-stranded RNA reveals that the RNA channel path is formed by two distinct features of the hDIS3 exoribonuclease: an open conformation and a domain organization more similar to bacterial RNase II than to yeast Rrp44. The cryo-EM structure of the holo-complex shows how obligate nuclear cofactors position the hMTR4 helicase at the entrance of the core complex, suggesting a striking structural conservation from lower to higher eukaryotes.

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Language(s): eng - English
 Dates: 2018-07
 Publication Status: Published online
 Pages: 22
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000440546000001
DOI: 10.7554/eLife.38686
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Title: eLife
Source Genre: Journal
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Publ. Info: Cambridge : eLife Sciences Publications
Pages: - Volume / Issue: 7 Sequence Number: e38686 Start / End Page: - Identifier: ISSN: 2050-084X
CoNE: https://pure.mpg.de/cone/journals/resource/2050-084X