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  mTORC1 Controls Phase Separation and the Biophysical Properties of the Cytoplasm by Tuning Crowding

Delarue, M., Brittingham, G. P., Pfeffer, S., Surovtsev, I. V., Pinglay, S., Kennedy, K. J., et al. (2018). mTORC1 Controls Phase Separation and the Biophysical Properties of the Cytoplasm by Tuning Crowding. Cell, 174(2), 338-349.e20. doi:10.1016/j.cell.2018.05.042.

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 Creators:
Delarue, M.1, Author
Brittingham, G. P.1, Author
Pfeffer, S.2, Author              
Surovtsev, I. V.1, Author
Pinglay, S.1, Author
Kennedy, K. J.1, Author
Schaffer, M.2, Author              
Gutierrez, J. I.1, Author
Sang, D.1, Author
Poterewicz, G.1, Author
Chung, J. K.1, Author
Plitzko, J. M.2, Author              
Groves, J. T.1, Author
Jacobs-Wagner, C.1, Author
Engel, B. D.2, Author              
Holt, L. J.1, Author
Affiliations:
1external, ou_persistent22              
2Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

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Free keywords: FLUORESCENCE CORRELATION SPECTROSCOPY; IN-SITU; LIVING CELLS; CRYOELECTRON TOMOGRAMS; SIGNAL-TRANSDUCTION; MEMBRANE-PROTEINS; IMAGE-ANALYSIS; BUDDING YEAST; PARTICLE; KINASEBiochemistry & Molecular Biology; Cell Biology;
 Abstract: Macromolecular crowding has a profound impact on reaction rates and the physical properties of the cell interior, but the mechanisms that regulate crowding are poorly understood. We developed genetically encoded multimeric nanoparticles (GEMs) to dissect these mechanisms. GEMs are homomultimeric scaffolds fused to a fluorescent protein that selfassemble into bright, stable particles of defined size and shape. By combining tracking of GEMs with genetic and pharmacological approaches, we discovered that the mTORC1 pathway can modulate the effective diffusion coefficient of particlesR20 nm in diameter more than 2-fold by tuning ribosome concentration, without any discernable effect on the motion of molecules% 5 nm. This change in ribosome concentration affected phase separation both in vitro and in vivo. Together, these results establish a role for mTORC1 in controlling both the mesoscale biophysical properties of the cytoplasm and biomolecular condensation.

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Language(s): eng - English
 Dates: 2018-06-212018
 Publication Status: Published in print
 Pages: 32
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

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Title: Cell
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 174 (2) Sequence Number: - Start / End Page: 338 - 349.e20 Identifier: ISSN: 0092-8674
CoNE: https://pure.mpg.de/cone/journals/resource/954925463183