English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Oscillatory Switches of Dorso-Ventral Polarity in Cells Confined between Two Surfaces

Helenius, J., Ecke, M., Mueller, D. J., & Gerisch, G. (2018). Oscillatory Switches of Dorso-Ventral Polarity in Cells Confined between Two Surfaces. Biophysical Journal, 115(1), 150-162. doi:10.1016/j.bpj.2018.05.025.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0002-039B-C Version Permalink: http://hdl.handle.net/21.11116/0000-0002-039C-B
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Helenius, Jonne1, Author
Ecke, Mary2, Author              
Mueller, Daniel J.1, Author
Gerisch, Günther2, Author              
Affiliations:
1external, ou_persistent22              
2Gerisch, Günther / Cell Dynamics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565149              

Content

show
hide
Free keywords: PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE; ACTIN ORGANIZATION; SELF-ORGANIZATION; TRAVELING-WAVES; PIP3 WAVES; DYNAMICS; MIGRATION; DICTYOSTELIUM; PHAGOCYTOSIS; CHEMOTAXISBiophysics;
 Abstract: To maneuver in a three-dimensional space, migrating cells need to accommodate to multiple surfaces. In particular, phagocytes have to explore their environment in the search for particles to be ingested. To examine how cells decide between competing surfaces, we exposed single cells of Dictyostelium to a defined three-dimensional space by confining them between two planar surfaces: those of a cover glass and of a wedged microcantilever. These cells form propagating waves of filamentous actin and PIP3 on their ventral substrate-attached surface. The dynamics of wave formation in the confined cells was explored using two-focus fluorescence imaging. When waves formed on one substrate, wave formation on the other substrate was efficiently suppressed. The propensity for wave formation switched between the opposing cell surfaces with periods of 2-5 min by one of two modes: 1) a rolling mode involving the slipping of a wave along the nonattached plasma membrane and 2) de novo initiation of waves on the previously blank cell surface. These data provide evidence for a cell-autonomous oscillator that switches dorso-ventral polarity in a cell simultaneously exposed to multiple substrate surfaces.

Details

show
hide
Language(s): eng - English
 Dates: 2018
 Publication Status: Published in print
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000437825800016
DOI: 10.1016/j.bpj.2018.05.025
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Biophysical Journal
  Other : Biophys. J.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 115 (1) Sequence Number: - Start / End Page: 150 - 162 Identifier: ISSN: 0006-3495
CoNE: https://pure.mpg.de/cone/journals/resource/954925385117