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  EASI-tag enables accurate multiplexed and interference-free MS2-based proteome quantification

Virreira Winter, S., Meier, F., Wichmann, C., Cox, J., Mann, M., & Meissner, F. (2018). EASI-tag enables accurate multiplexed and interference-free MS2-based proteome quantification. Nature methods, 15(7), 527-530. doi:10.1038/s41592-018-0037-8.

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 Creators:
Virreira Winter, Sebastian1, Author              
Meier, Florian2, Author              
Wichmann, Christoph3, Author              
Cox, Juergen3, Author              
Mann, Matthias2, Author              
Meissner, Felix1, Author              
Affiliations:
1Meissner, Felix / Experimental Systems Immunology, Max Planck Institute of Biochemistry, Max Planck Society, ou_2149678              
2Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              
3Cox, Jürgen / Computational Systems Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society, ou_2063284              

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Free keywords: IDENTIFICATION RATES; MASS-SPECTROMETRY; LTQ ORBITRAP; PURIFICATION; STRATEGY; BIOLOGY; CELLSBiochemistry & Molecular Biology;
 Abstract: We developed EASI-tag (easily abstractable sulfoxide-based isobaric-tag), a new type of amine-derivatizing and sulfoxide- containing isobaric labeling reagents for highly accurate quantitative proteomics analysis using mass spectrometry. We observed that EASI-tag labels dissociate at low collision energy and generate peptide-coupled, interference-free reporter ions with high yield. Efficient isolation of C-12 precursors and quantification at the MS2 level allowed accurate determination of quantitative differences between up to six multiplexed samples.

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Language(s): eng - English
 Dates: 2018
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000437934800018
DOI: 10.1038/s41592-018-0037-8
 Degree: -

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Project name : MSmed project (F.M., P.E.G., S.V.W., J.C., M.M.)
Grant ID : 686547
Funding program : Horizon 2020 (H2020)
Funding organization : European Commission (EC)

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Title: Nature methods
  Other : Nature methods
Source Genre: Journal
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Publ. Info: New York, NY : Nature Pub. Group
Pages: - Volume / Issue: 15 (7) Sequence Number: - Start / End Page: 527 - 530 Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556