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  Pleomorphic linkers as ubiquitous structural organizers of vesicles in axons

Schrod, N., Vanhecke, D., Laugks, U., Stein, V., Fukuda, Y., Schaffer, M., et al. (2018). Pleomorphic linkers as ubiquitous structural organizers of vesicles in axons. PLoS One, 13(6): e0197886. doi:10.1371/journal.pone.0197886.

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journal.pone.0197886.pdf (Publisher version), 26MB
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 Creators:
Schrod, Nikolas1, Author           
Vanhecke, Dimitri1, Author           
Laugks, Ulrike1, Author           
Stein, Valentin2, Author
Fukuda, Yoshiyuki1, Author           
Schaffer, Miroslava1, Author           
Baumeister, Wolfgang1, Author           
Lucic, Vladan1, Author           
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1Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              
2external, ou_persistent22              

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Free keywords: PRESYNAPTIC ACTIVE ZONE; DENSE-CORE VESICLES; CRYOELECTRON TOMOGRAPHY REVEALS; CULTURED HIPPOCAMPAL-NEURONS; SMOOTH ENDOPLASMIC-RETICULUM; CENTRAL-NERVOUS-SYSTEM; SYNAPTIC VESICLES; ELECTRON TOMOGRAPHY; QUANTITATIVE-ANALYSIS; TRANSPORT VESICLESScience & Technology - Other Topics;
 Abstract: Many cellular processes depend on a precise structural organization of molecular components. Here, we established that neurons grown in culture provide a suitable system for in situ structural investigations of cellular structures by cryo-electron tomography, a method that allows high resolution, three-dimensional imaging of fully hydrated, vitrified cellular samples. A higher level of detail of cellular components present in our images allowed us to quantitatively characterize presynaptic and cytoskeletal organization, as well as structures involved in axonal transport and endocytosis. In this way we provide a structural framework into which information from other methods need to fit. Importantly, we show that short pleomorphic linkers (tethers and connectors) extensively interconnect different types of spherical vesicles and other lipid membranes in neurons imaged in a close-to-native state. These linkers likely serve to organize and precisely position vesicles involved in endocytosis, axonal transport and synaptic release. Hence, structural interactions via short linkers may serve as ubiquitous vesicle organizers in neuronal cells.

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Language(s): eng - English
 Dates: 2018-06
 Publication Status: Published online
 Pages: 24
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: PLoS One
Source Genre: Journal
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Publ. Info: San Francisco, CA : Public Library of Science
Pages: - Volume / Issue: 13 (6) Sequence Number: e0197886 Start / End Page: - Identifier: ISSN: 1932-6203
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000277850