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  Cryo-EM of nucleosome core particle interactions in trans

Bilokapic, S., Strauss, M., & Halic, M. (2018). Cryo-EM of nucleosome core particle interactions in trans. Scientific Reports, 8: 7046. doi:10.1038/s41598-018-25429-1.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-A4DC-D Version Permalink: http://hdl.handle.net/21.11116/0000-0002-A4DD-C
Genre: Journal Article

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License.
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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License

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 Creators:
Bilokapic, Silvija1, Author
Strauss, Mike2, Author              
Halic, Mario1, Author
Affiliations:
1external, ou_persistent22              
2Scientific Service Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565170              

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Free keywords: 30-NM CHROMATIN FIBER; H3 TAIL DOMAIN; HISTONE OCTAMER; CRYSTAL-STRUCTURE; STRUCTURAL BASIS; IN-VIVO; DNA; CHROMOSOMES; GENOME; ARRAYSScience & Technology - Other Topics;
 Abstract: Nucleosomes, the basic unit of chromatin, are repetitively spaced along DNA and regulate genome expression and maintenance. The long linear chromatin molecule is extensively condensed to fit DNA inside the nucleus. How distant nucleosomes interact to build tertiary chromatin structure remains elusive. In this study, we used cryo-EM to structurally characterize different states of long range nucleosome core particle (NCP) interactions. Our structures show that NCP pairs can adopt multiple conformations, but, commonly, two NCPs are oriented with the histone octamers facing each other. In this conformation, the dyad of both nucleosome core particles is facing the same direction, however, the NCPs are laterally shifted and tilted. The histone octamer surface and histone tails in trans NCP pairs remain accessible to regulatory proteins. The overall conformational flexibility of the NCP pair suggests that chromatin tertiary structure is dynamic and allows access of various chromatin modifying machineries to nucleosomes.

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Language(s): eng - English
 Dates: 2018
 Publication Status: Published online
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
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Title: Scientific Reports
  Abbreviation : Sci. Rep.
Source Genre: Journal
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Publ. Info: London, UK : Nature Publishing Group
Pages: - Volume / Issue: 8 Sequence Number: 7046 Start / End Page: - Identifier: ISSN: 2045-2322
CoNE: https://pure.mpg.de/cone/journals/resource/2045-2322