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  Sulfide Protects [FeFe] Hydrogenases From O2

Rodríguez-Maciá, P., Reijerse, E. J., van Gastel, M., DeBeer, S., Lubitz, W., Rüdiger, O., et al. (2018). Sulfide Protects [FeFe] Hydrogenases From O2. Journal of the American Chemical Society, 140(30), 9346-9350. doi:10.1021/jacs.8b04339.

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 Creators:
Rodríguez-Maciá, Patricia1, Author
Reijerse, Edward J.1, Author
van Gastel, Maurice2, Author              
DeBeer, Serena1, Author
Lubitz, Wolfgang1, Author
Rüdiger, Olaf1, Author
Birrell, James A.1, Author
Affiliations:
1Max Planck Institute for Chemical Energy Conversion, Stiftstraße 34-36, D-45470 Mülheim an der Ruhr, Germany, ou_persistent22              
2Research Group van Gastel, Max-Planck-Institut für Kohlenforschung, Max Planck Society, ou_2541713              

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 Abstract: [FeFe] hydrogenases catalyze proton reduction and hydrogen oxidation with high rates and efficiency under physiological conditions, but are highly oxygen sensitive. The [FeFe] hydrogenase from Desulfovibrio desulfuricans (DdHydAB) can be purified under air in an oxygen stable inactive state Hoxair. The formation of the Hoxair state in vitro allows the handling of hydrogenases in air, making their implementation in biotechnological applications more feasible. Here, we report a simple and robust protocol for the formation of the Hoxair state in DdHydAB and the [FeFe] hydrogenase from Chlamydomonas reinhardtii, which is based on high potential inactivation in the presence of sulfide.

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Language(s): eng - English
 Dates: 2018-04-272018-07-162018-08-01
 Publication Status: Published in print
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1021/jacs.8b04339
 Degree: -

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Title: Journal of the American Chemical Society
  Other : J. Am. Chem. Soc.
  Abbreviation : JACS
Source Genre: Journal
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Publ. Info: Washington, DC : American Chemical Society
Pages: - Volume / Issue: 140 (30) Sequence Number: - Start / End Page: 9346 - 9350 Identifier: ISSN: 0002-7863
CoNE: https://pure.mpg.de/cone/journals/resource/954925376870