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  Structure of activated transcription complex Pol II-DSIF-PAF-SPT6.

Vos, S. M., Farnung, L., Böhning, M., Wigge, C., Linden, A., Urlaub, H., et al. (2018). Structure of activated transcription complex Pol II-DSIF-PAF-SPT6. Nature, 560(7720), 607-612. doi:10.1038/s41586-018-0440-4.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-FF66-E Version Permalink: http://hdl.handle.net/21.11116/0000-0003-C001-2
Genre: Journal Article

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 Creators:
Vos, S. M.1, Author              
Farnung, L.1, Author              
Böhning, M.1, Author              
Wigge, C.1, Author              
Linden, A.2, Author              
Urlaub, H.2, Author              
Cramer, P.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              
2Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              

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 Abstract: Gene regulation involves activation of RNA polymerase II (Pol II) that is paused and bound by the protein complexes DRB sensitivity-inducing factor (DSIF) and negative elongation factor (NELF). Here we show that formation of an activated Pol II elongation complex in vitro requires the kinase function of the positive transcription elongation factor b (P-TEFb) and the elongation factors PAF1 complex (PAF) and SPT6. The cryo-EM structure of an activated elongation complex of Sus scrofa Pol II and Homo sapiens DSIF, PAF and SPT6 was determined at 3.1 Å resolution and compared to the structure of the paused elongation complex formed by Pol II, DSIF and NELF. PAF displaces NELF from the Pol II funnel for pause release. P-TEFb phosphorylates the Pol II linker to the C-terminal domain. SPT6 binds to the phosphorylated C-terminal-domain linker and opens the RNA clamp formed by DSIF. These results provide the molecular basis for Pol II pause release and elongation activation.

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Language(s): eng - English
 Dates: 2018-08-222018-08-30
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/s41586-018-0440-4
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Title: Nature
Source Genre: Journal
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Pages: - Volume / Issue: 560 (7720) Sequence Number: - Start / End Page: 607 - 612 Identifier: -