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  Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion

Nekrasov, V., Wang, C., Win, J., Lanz, C., Weigel, D., & Kamoun, S. (2017). Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion. Scientific Reports, 7: 482. doi:10.1038/s41598-017-00578-x.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0002-04E9-3 Version Permalink: https://hdl.handle.net/21.11116/0000-0002-04EA-2
Genre: Journal Article

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https://www.nature.com/articles/s41598-017-00578-x (Publisher version)
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 Creators:
Nekrasov, Vladimir1, Author
Wang, Congmao2, Author
Win, Joe1, Author
Lanz, Christa3, Author
Weigel, Detlef3, Author
Kamoun, Sophien1, Author
Affiliations:
1The Sainsbury Laboratory, Norwich Research Park, Norwich, ou_persistent22              
2Institute of Digital Agriculture, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, ou_persistent22              
3Max Planck Institute for Developmental Biology, Max Planck Society, Max-Planck-Ring 5, 72076 Tübingen, DE, ou_2421691              

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Free keywords: EDITED CROPS; GENE; IMMUNITY
 Abstract: Genome editing has emerged as a technology with a potential to revolutionize plant breeding. In this study, we report on generating, in less than ten months, Tomelo, a non- transgenic tomato variety resistant to the powdery mildew fungal pathogen using the CRISPR/ Cas9 technology. We used wholegenome sequencing to show that Tomelo does not carry any foreign DNA sequences but only carries a deletion that is indistinguishable from naturally occurring mutations. We also present evidence for CRISPR/ Cas9 being a highly precise tool, as we did not detect off- target mutations in Tomelo. Using our pipeline, mutations can be readily introduced into elite or locally adapted tomato varieties in less than a year with relatively minimal effort and investment.

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Language(s): eng - English
 Dates: 2017-02-162017-02-222017-03-28
 Publication Status: Published online
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000397528600014
DOI: 10.1038/s41598-017-00578-x
 Degree: -

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Title: Scientific Reports
  Abbreviation : Sci. Rep.
Source Genre: Journal
 Creator(s):
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Publ. Info: London, UK : Nature Publishing Group
Pages: - Volume / Issue: 7 Sequence Number: 482 Start / End Page: - Identifier: ISSN: 2045-2322
CoNE: https://pure.mpg.de/cone/journals/resource/2045-2322