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  Assessing Distribution and Variation of Genome-Wide DNA Methylation Using Short-Read Sequencing

Hagmann, J., & Becker, C. (2017). Assessing Distribution and Variation of Genome-Wide DNA Methylation Using Short-Read Sequencing. Methods in Molecular Biology, 1610. doi:10.1007/978-1-4939-7003-2_5.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0002-04B6-C Version Permalink: https://hdl.handle.net/21.11116/0000-000A-7AB4-2
Genre: Journal Article

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 Creators:
Hagmann, J, Author           
Becker, C1, Author           
Affiliations:
1Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society, ou_3375790              

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Free keywords: ARABIDOPSIS; DNA methylation; Short-read sequencing; Bisulfite; Differential methylation; Differentially methylated region;
 Abstract: DNA methylation is one of the most prominent epigenetic marks and is particularly complex in plant genomes. Whole-genome bisulfite sequencing (WGBS) using short reads has become the standard tool to study genome-wide patterns of DNA methylation. The goal of the present protocol is to enable readers to perform WGBS on both the wet lab and the computational side. We briefly outline important steps in bisulfite library preparation, then focus on the different aspects of DNA methylation analysis, from read mapping to identifying biologically relevant differential methylation between different samples.

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Language(s): eng - English
 Dates: 2017
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1007/978-1-4939-7003-2_5
PMID: 28439857
 Degree: -

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Title: Methods in Molecular Biology
  Other : Methods Mol. Biol.
Source Genre: Series
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Publ. Info: US : Springer
Pages: - Volume / Issue: 1610 Sequence Number: - Start / End Page: - Identifier: ISSN: 1064-3745
CoNE: https://pure.mpg.de/cone/journals/resource/954927725544

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Title: Plant Genomics: Methods and Protocols
Source Genre: Book
 Creator(s):
Busch, W, Editor
Affiliations:
-
Publ. Info: New York, NY, USA : Humana Press
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 61 - 72 Identifier: ISBN: 978-1-4939-7001-8
DOI: 10.1007/978-1-4939-7003-2