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  Replication of bacterial plasmids in the nucleus of the red alga Porphyridium purpureum

Li, Z., & Bock, R. (2018). Replication of bacterial plasmids in the nucleus of the red alga Porphyridium purpureum. Nature Communications, 9(1): 3451. doi:10.1038/s41467-018-05651-1.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-0570-A Version Permalink: http://hdl.handle.net/21.11116/0000-0002-0571-9
Genre: Journal Article

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 Creators:
Li, Z.1, Author              
Bock, R.1, Author              
Affiliations:
1Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753326              

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 Abstract: Rhodophytes (red algae) are a diverse group of algae with great ecological and economic importance. However, tools for post-genomic research on red algae are still largely lacking. Here, we report the development of an efficient genetic transformation system for the model rhodophyte Porphyridium purpureum. We show that transgenes can be expressed to unprecedented levels of up to 5% of the total soluble protein. Surprisingly, the transgenic DNA is maintained episomally, as extrachromosomal high-copy number plasmid. The bacterial replication origin confers replication in the algal nucleus, thus providing an intriguing example of a prokaryotic replication origin functioning in a eukaryotic system. The extended presence of bacterial episomal elements may provide an evolutionary explanation for the frequent natural occurrence of extrachromosomal plasmids in red algae, and may also have contributed to the high rate of horizontal gene transfer from bacteria to the nuclear genome of Porphyridium purpureum and other rhodophytes.

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Language(s): eng - English
 Dates: 2018
 Publication Status: Published in print
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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 9 (1) Sequence Number: 3451 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723