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  Megahertz data collection from protein microcrystals at an X-ray free-electron laser

Grünbein, M. L., Bielecki, J., Gorel, A., Stricker, M., Bean, R., Cammarata, M., et al. (2018). Megahertz data collection from protein microcrystals at an X-ray free-electron laser. Nature Communications, 9(1): 3487, pp. 1-9. doi:10.1038/s41467-018-05953-4.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-05B2-F Version Permalink: http://hdl.handle.net/21.11116/0000-0002-C0DB-E
Genre: Journal Article

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 Creators:
Grünbein, Marie Luise1, Author              
Bielecki, Johan, Author
Gorel, Alexander1, Author              
Stricker, Miriam1, Author              
Bean, Richard, Author
Cammarata, Marco, Author
Dörner, Katerina, Author
Fröhlich, Lars, Author
Hartmann, Elisabeth1, Author              
Hauf, Steffen, Author
Hilpert, Mario1, Author              
Kim, Yoonhee, Author
Kloos, Marco (Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society), Marco, Author
Letrun, Romain, Author
Messerschmidt, Marc, Author
Mills, Grant, Author
Nass Kovács, Gabriela1, Author              
Ramilli, Marco, Author
Roome, Christopher M.1, Author              
Sato, Tokushi, Author
Scholz, Matthias, AuthorSliwa, Michel, AuthorSztuk-Dambietz, Jolanta, AuthorWeik, Martin, AuthorWeinhausen, Britta, AuthorAl-Qudami, Nasser, AuthorBoukhelef, Djelloul, AuthorBrockhauser, Sandor, AuthorEhsan, Wajid, AuthorEmons, Moritz, AuthorEsenov, Sergey, AuthorFangohr, Hans, AuthorKaukher, Alexander, AuthorKluyver, Thomas, AuthorLederer, Max, AuthorMaia, Luis, AuthorManetti, Maurizio, AuthorMichelat, Thomas, AuthorMünnich, Astrid, AuthorPallas, Florent, AuthorPalmer, Guido, AuthorPrevitali, Gianpietro, AuthorRaab, Natascha, AuthorSilenzi, Alessandro, AuthorSzuba, Janusz, AuthorVenkatesan, Sandhya, AuthorWrona, Krzysztof, AuthorZhu, Jun, AuthorDoak, Bruce1, Author              Shoeman, Robert L.1, Author              Foucar, Lutz1, Author              Colletier, Jacques-Philippe, AuthorMancuso, Adrian P., AuthorBarends, Thomas R. M.1, Author              Stan, Claudiu A., AuthorSchlichting, Ilme1, Author               more..
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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Free keywords: Free-electron lasers ; Nanocrystallography
 Abstract: X-ray free-electron lasers (XFELs) enable novel experiments because of their high peak brilliance and femtosecond pulse duration. However, non-superconducting XFELs offer repetition rates of only 10–120 Hz, placing significant demands on beam time and sample consumption. We describe serial femtosecond crystallography experiments performed at the European XFEL, the first MHz repetition rate XFEL, delivering 1.128 MHz X-ray pulse trains at 10 Hz. Given the short spacing between pulses, damage caused by shock waves launched by one XFEL pulse on sample probed by subsequent pulses is a concern. To investigate this issue, we collected data from lysozyme microcrystals, exposed to a ~15 μm XFEL beam. Under these conditions, data quality is independent of whether the first or subsequent pulses of the train were used for data collection. We also analyzed a mixture of microcrystals of jack bean proteins, from which the structure of native, magnesium-containing concanavalin A was determined.

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Language(s): eng - English
 Dates: 2018-07-112018-08-012018-08-28
 Publication Status: Published online
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 9 (1) Sequence Number: 3487 Start / End Page: 1 - 9 Identifier: ISSN: 2041-1723
CoNE: /journals/resource/2041-1723