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  Correlated light and electron microscopy of cell division in large marine oocytes, eggs and embryos.

Burdyniuk, M., Wesolowska, N., Fleszar, M., Karreman, M. A., Machado, P., Borrego-Pinto, J., et al. (2018). Correlated light and electron microscopy of cell division in large marine oocytes, eggs and embryos. In H. Maiato, & M. Schuh (Eds.), Mitosis and Meiosis Part B (pp. 293-313). doi:10.1016/bs.mcb.2018.03.031.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-0BF7-C Version Permalink: http://hdl.handle.net/21.11116/0000-0002-107C-1
Genre: Book Chapter

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2640089.pdf (Publisher version), 3MB
 
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 Creators:
Burdyniuk, M., Author
Wesolowska, N., Author
Fleszar, M., Author
Karreman, M. A., Author
Machado, P., Author
Borrego-Pinto, J., Author
Ruthensteiner, B., Author
Schwab, Y., Author
Lenart, P.1, Author              
Affiliations:
1Research Group of Cytoskeletal Dynamics in Oocytes, MPI for Biophysical Chemistry, Max Planck Society, ou_2640691              

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Free keywords: Starfish oocyte meiosis; Cell division; Correlative light and electron microscopy; Cytoskeleton live-cell imaging
 Abstract: The rapid and synchronous divisions of large and transparent oocytes, eggs, and embryos of marine species are exceptionally well suited for microscopic observation. Consequently, these cells have been models for cell division research since its beginnings and contributed some of its first and most fundamental discoveries. While large size and rapid transitions render these cells ideal specimens for light microscopy, the same features constitute a challenge for electron microscopy. Here, we describe example protocols from our work on starfish oocyte meiosis, where we overcome these challenges by using live imaging of fluorescently labeled structures in combination with correlated electron microscopy. In this work, we demonstrate how: (i) to capture a rapid, transient event in time and (ii) to localize a small structure within the large oocyte. These techniques are applicable with minor modifications to oocytes and embryos of other species and, possibly, to other cell types.

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Language(s): eng - English
 Dates: 2018-04-262018
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/bs.mcb.2018.03.031
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Title: Mitosis and Meiosis Part B
Source Genre: Book
 Creator(s):
Maiato, H., Editor
Schuh, M., Editor
Affiliations:
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Publ. Info: -
Pages: - Volume / Issue: 145 Sequence Number: - Start / End Page: 293 - 313 Identifier: ISBN: 978-0-12-814142-7

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Title: Methods in Cell Biology
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Publ. Info: Elsevier
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: -