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  F-Actin nucleated on chromosomes coordinates their capture by microtubules in oocyte meiosis.

Burdyniuk, M., Callegari, A., Mori, M., Nédélec, F., & Lenart, P. (2018). F-Actin nucleated on chromosomes coordinates their capture by microtubules in oocyte meiosis. Journal of Cell Biology, 217(8), 2661-2674. doi:10.1083/jcb.201802080.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-0C0E-3 Version Permalink: http://hdl.handle.net/21.11116/0000-0002-0C9F-F
Genre: Journal Article

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Burdyniuk, M., Author
Callegari, A., Author
Mori, M., Author
Nédélec, F., Author
Lenart, P.1, Author              
Affiliations:
1Research Group of Cytoskeletal Dynamics in Oocytes, MPI for Biophysical Chemistry, Max Planck Society, ou_2640691              

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 Abstract: Capture of each and every chromosome by spindle microtubules is essential to prevent chromosome loss and aneuploidy. In somatic cells, astral microtubules search and capture chromosomes forming lateral attachments to kinetochores. However, this mechanism alone is insufficient in large oocytes. We have previously shown that a contractile F-actin network is additionally required to collect chromosomes scattered in the 70-µm starfish oocyte nucleus. How this F-actin–driven mechanism is coordinated with microtubule capture remained unknown. Here, we show that after nuclear envelope breakdown Arp2/3-nucleated F-actin “patches” form around chromosomes in a Ran-GTP–dependent manner, and we propose that these structures sterically block kinetochore–microtubule attachments. Once F-actin–driven chromosome transport is complete, coordinated disassembly of F-actin patches allows synchronous capture by microtubules. Our observations indicate that this coordination is necessary because early capture of chromosomes by microtubules would interfere with F-actin–driven transport leading to chromosome loss and formation of aneuploid eggs.

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Language(s): eng - English
 Dates: 2018-06-142018-08-06
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1083/jcb.201802080
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Title: Journal of Cell Biology
Source Genre: Journal
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Pages: - Volume / Issue: 217 (8) Sequence Number: - Start / End Page: 2661 - 2674 Identifier: -