English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Analysis of cytokinesis by electron microscopy.

König, J., Borrego-Pinto, J., Streichert, D., Munzig, M., Lenart, P., & Müller-Reichert, T. (2017). Analysis of cytokinesis by electron microscopy. In A. Echard (Ed.), Cytokinesis (pp. 225-238). Elsevier. doi:10.1016/bs.mcb.2016.03.029.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0002-0CBF-B Version Permalink: http://hdl.handle.net/21.11116/0000-0002-1078-5
Genre: Book Chapter

Files

show Files
hide Files
:
2640183.pdf (Publisher version), 803KB
 
File Permalink:
-
Name:
2640183.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Biophysical Chemistry (Karl Friedrich Bonhoeffer Institute), MBPC; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
König, J., Author
Borrego-Pinto, J., Author
Streichert, D., Author
Munzig, M., Author
Lenart, P.1, Author              
Müller-Reichert, T., Author
Affiliations:
1Research Group of Cytoskeletal Dynamics in Oocytes, MPI for Biophysical Chemistry, Max Planck Society, ou_2640691              

Content

show
hide
Free keywords: Abscission; Correlative light electron microscopy; Cytokinesis; Electron microscopy; Electron tomography; High-pressure freezing
 Abstract: Following up on a chapter on the Correlative Light and Electron Microscopy of Early Caenorhabditis elegans Embryos in Mitosis (MCB 79, 101-119), we present an adaptation of our established protocol for the ultrastructural analysis of either permeabilized or injected embryonic systems. We prepared both drug-treated early C. elegans embryos and fluorescently labeled sea urchin embryos of Lytechinus pictus for ultrastructural studies on animal cytokinesis. Here we focus on the initial preparation steps of postmitotic embryos for high-pressure freezing and subsequent electron microscopy with an emphasis on electron tomography. The advantages and limitations of our extended protocol will be discussed.

Details

show
hide
Language(s): eng - English
 Dates: 2016-06-282017
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/bs.mcb.2016.03.029
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Cytokinesis
Source Genre: Book
 Creator(s):
Echard, A., Editor
Affiliations:
-
Publ. Info: Elsevier
Pages: - Volume / Issue: 137 Sequence Number: - Start / End Page: 225 - 238 Identifier: ISBN: 978-0-12-809673-4

Source 2

show
hide
Title: Methods in Cell Biology
Source Genre: Series
 Creator(s):
Affiliations:
Publ. Info: Elsevier
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: -