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  The spectraplakin Short stop is an essential microtubule regulator involved in epithelial closure in Drosophila.

Takács, Z., Jankovics, F., Vilmos, P., Lenart, P., Röper, K., & Erdélyi, M. (2017). The spectraplakin Short stop is an essential microtubule regulator involved in epithelial closure in Drosophila. Journal of Cell Science, 130(4), 712-724. doi:10.1242/jcs.193003.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-0CDA-C Version Permalink: http://hdl.handle.net/21.11116/0000-0002-0CDF-7
Genre: Journal Article

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 Creators:
Takács, Z., Author
Jankovics, F., Author
Vilmos, P., Author
Lenart, P.1, Author              
Röper, K., Author
Erdélyi, M., Author
Affiliations:
1Research Group of Cytoskeletal Dynamics in Oocytes, MPI for Biophysical Chemistry, Max Planck Society, ou_2640691              

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Free keywords: Actin; Dorsal closure; Drosophila; Microtubule; Short stop; Spectraplakin
 Abstract: Dorsal closure of the Drosophila embryonic epithelium provides an excellent model system for the in vivo analysis of molecular mechanisms regulating cytoskeletal rearrangements. In this study, we investigated the function of the Drosophila spectraplakin Short stop (Shot), a conserved cytoskeletal structural protein, during closure of the dorsal embryonic epithelium. We show that Shot is essential for the efficient final zippering of the opposing epithelial margins. By using isoform-specific mutant alleles and genetic rescue experiments with truncated Shot variants, we demonstrate that Shot functions as an actin-microtubule cross-linker in mediating zippering. At the leading edge of epithelial cells, Shot regulates protrusion dynamics by promoting filopodia formation. Fluorescence recovery after photobleaching (FRAP) analysis and in vivo imaging of microtubule growth revealed that Shot stabilizes dynamic microtubules. The actin- and microtubule-binding activities of Shot are simultaneously required in the same molecule, indicating that Shot is engaged as a physical crosslinker in this process. We propose that Shot-mediated interactions between microtubules and actin filaments facilitate filopodia formation, which promotes zippering by initiating contact between opposing epithelial cells.

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Language(s): eng - English
 Dates: 2017-02-15
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1242/jcs.193003
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Title: Journal of Cell Science
Source Genre: Journal
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Pages: - Volume / Issue: 130 (4) Sequence Number: - Start / End Page: 712 - 724 Identifier: -