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  Live imaging of centriole dynamics by fluorescently tagged proteins in starfish oocyte meiosis.

Borrego-Pinto, J., Somogy, K., & Lenart, P. (2016). Live imaging of centriole dynamics by fluorescently tagged proteins in starfish oocyte meiosis. In I. P. Nezis (Ed.), Oogenesis (pp. 145-166). Springer-Verlag.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-0D6B-9 Version Permalink: http://hdl.handle.net/21.11116/0000-0002-1077-6
Genre: Book Chapter

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2640285.pdf (Publisher version), 844KB
 
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 Creators:
Borrego-Pinto, J., Author
Somogy, K., Author
Lenart, P.1, Author              
Affiliations:
1Research Group of Cytoskeletal Dynamics in Oocytes, MPI for Biophysical Chemistry, Max Planck Society, ou_2640691              

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 Abstract: High throughput DNA sequencing, the decreasing costs of DNA synthesis, and universal techniques for genetic manipulation have made it much easier and quicker to establish molecular tools for any organism than it has been 5 years ago. This opens a great opportunity for reviving “nonconventional” model organisms, which are particularly suited to study a specific biological process and many of which have already been established before the era of molecular biology. By taking advantage of transcriptomics, in particular, these systems can now be easily turned into full fetched models for molecular cell biology. As an example, here we describe how we established molecular tools in the starfish Patiria miniata, which has been a popular model for cell and developmental biology due to the synchronous and rapid development, transparency, and easy handling of oocytes, eggs, and embryos. Here, we detail how we used a de novo assembled transcriptome to produce molecular markers and established conditions for live imaging to investigate the molecular mechanisms underlying centriole elimination—a poorly understood process essential for sexual reproduction of animal species.

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Language(s): eng - English
 Dates: 2016
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1007%2F978-1-4939-3795-0_11
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Title: Oogenesis
Source Genre: Book
 Creator(s):
Nezis, I. P., Editor
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Publ. Info: Springer-Verlag
Pages: - Volume / Issue: 1457 Sequence Number: - Start / End Page: 145 - 166 Identifier: -

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Title: Methods in Molecular Biology
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