Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
  Imaging neuronal population activity in awake and anesthetized rodents

Greenberg, D., Wallace, D., & Kerr, J. (2014). Imaging neuronal population activity in awake and anesthetized rodents. Cold Spring Harbor Protocols, 2014(9), 912-922. doi:10.1101/pdb.top083535.

Item is

Basisdaten

einblenden: ausblenden:
Genre: Zeitschriftenartikel

Externe Referenzen

einblenden:
ausblenden:
Beschreibung:
-
OA-Status:

Urheber

einblenden:
ausblenden:
 Urheber:
Greenberg, DS1, 2, 3, Autor           
Wallace, DJ1, 2, 3, Autor           
Kerr, JND1, 2, 3, Autor           
Affiliations:
1Former Research Group Network Imaging, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_2528697              
2Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497794              
3Research Group Neural Population Imaging, Max Planck Institute for Biological Cybernetics, Max Planck Society, Spemannstrasse 38, 72076 Tübingen, DE, ou_1497807              

Inhalt

einblenden:
ausblenden:
Schlagwörter: -
 Zusammenfassung: Recent advances in in vivo two-photon imaging have extended the technique to permit the detection of action potentials (APs) in populations of spatially resolved neurons in awake animals. Although experimentally demanding, this technique's potential applications include experiments to investigate perception, behavior, and other awake states. Here we outline experimental procedures for imaging neuronal populations in awake and anesthetized rodents. Details are provided on habituation to head fixation, surgery, head plate design, and dye injection. Determination of AP detection accuracy through simultaneous optical and electrophysiological recordings is also discussed. Basic problems of data analysis are considered, such as correction of signal background and baseline drift, AP detection, and motion correction. As an application of the method, the comparison of neuronal activity across arousal states is considered in detail, and some future directions are discussed.

Details

einblenden:
ausblenden:
Sprache(n):
 Datum: 2014-09
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: DOI: 10.1101/pdb.top083535
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Cold Spring Harbor Protocols
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 2014 (9) Artikelnummer: - Start- / Endseite: 912 - 922 Identifikator: -