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  Distinct mechanisms eliminate mother and daughter centrioles in meiosis of starfish oocytes.

Borrego-Pinto, J., Somogyi, K., Karreman, M. A., König, J., Müller-Reichert, T., Bettencourt-Dias, M., et al. (2016). Distinct mechanisms eliminate mother and daughter centrioles in meiosis of starfish oocytes. Journal of Cell Biology, 212(7): 815. doi:10.1083/jcb.201510083.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-0E65-E Version Permalink: http://hdl.handle.net/21.11116/0000-0002-0E6A-9
Genre: Journal Article

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Borrego-Pinto, J., Author
Somogyi, K., Author
Karreman, M. A., Author
König, J., Author
Müller-Reichert, T., Author
Bettencourt-Dias, M., Author
Gönczy, P., Author
Schwab, Y., Author
Lenart, P.1, Author              
Affiliations:
1Research Group of Cytoskeletal Dynamics in Oocytes, MPI for Biophysical Chemistry, Max Planck Society, ou_2640691              

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 Abstract: Centriole elimination is an essential process that occurs in female meiosis of metazoa to reset centriole number in the zygote at fertilization. How centrioles are eliminated remains poorly understood. Here we visualize the entire elimination process live in starfish oocytes. Using specific fluorescent markers, we demonstrate that the two older, mother centrioles are selectively removed from the oocyte by extrusion into polar bodies. We show that this requires specific positioning of the second meiotic spindle, achieved by dynein-driven transport, and anchorage of the mother centriole to the plasma membrane via mother-specific appendages. In contrast, the single daughter centriole remaining in the egg is eliminated before the first embryonic cleavage. We demonstrate that these distinct elimination mechanisms are necessary because if mother centrioles are artificially retained, they cannot be inactivated, resulting in multipolar zygotic spindles. Thus, our findings reveal a dual mechanism to eliminate centrioles: mothers are physically removed, whereas daughters are eliminated in the cytoplasm, preparing the egg for fertilization.

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Language(s): eng - English
 Dates: 2016-03-21
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.1083/jcb.201510083
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Title: Journal of Cell Biology
Source Genre: Journal
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Pages: 13 Volume / Issue: 212 (7) Sequence Number: 815 Start / End Page: - Identifier: -