English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  An Arp2/3 nucleated F-actin shell fragments nuclear membranes at nuclear envelope breakdown in starfish oocytes.

Mori, M., Somogy, K., Kondo, H., Monnier, N., Falk, H. J., Machado, P., et al. (2014). An Arp2/3 nucleated F-actin shell fragments nuclear membranes at nuclear envelope breakdown in starfish oocytes. Current Biology, 24(12), 1421-1428. doi:10.1016/j.cub.2014.05.019.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0002-0E89-5 Version Permalink: http://hdl.handle.net/21.11116/0000-0002-0E8F-F
Genre: Journal Article

Files

show Files
hide Files
:
2640419.pdf (Publisher version), 5MB
Name:
2640419.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2640419_Suppl.htm (Supplementary material), 65KB
Name:
2640419_Suppl.htm
Description:
-
Visibility:
Public
MIME-Type / Checksum:
text/html / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Mori, M., Author
Somogy, K., Author
Kondo, H., Author
Monnier, N., Author
Falk, H. J., Author
Machado, P., Author
Bathe, M., Author
Nédélec, F., Author
Lenart, P.1, Author              
Affiliations:
1Research Group of Cytoskeletal Dynamics in Oocytes, MPI for Biophysical Chemistry, Max Planck Society, ou_2640691              

Content

show
hide
Free keywords: -
 Abstract: Animal cells disassemble and reassemble their nuclear envelopes (NEs) upon each division. Nuclear envelope breakdown (NEBD) serves as a major regulatory mechanism by which mixing of cytoplasmic and nuclear compartments drives the complete reorganization of cellular architecture, committing the cell for division. Breakdown is initiated by phosphorylation-driven partial disassembly of the nuclear pore complexes (NPCs), increasing their permeability but leaving the overall NE structure intact. Subsequently, the NE is rapidly broken into membrane fragments, defining the transition from prophase to prometaphase and resulting in complete mixing of cyto- and nucleoplasm. However, the mechanism underlying this rapid NE fragmentation remains largely unknown. Here, we show that NE fragmentation during NEBD in starfish oocytes is driven by an Arp2/3 complex-nucleated F-actin "shell" that transiently polymerizes on the inner surface of the NE. Blocking the formation of this F-actin shell prevents membrane fragmentation and delays entry of large cytoplasmic molecules into the nucleus. We observe spike-like protrusions extending from the F-actin shell that appear to "pierce" the NE during the fragmentation process. Finally, we show that NE fragmentation is essential for successful reproduction, because blocking this process in meiosis leads to formation of aneuploid eggs.

Details

show
hide
Language(s): eng - English
 Dates: 2014-06-052014-06-16
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.cub.2014.05.019
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Current Biology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 24 (12) Sequence Number: - Start / End Page: 1421 - 1428 Identifier: -