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  Intracellular transport by an anchored homogeneously contracting F-actin meshwork.

Mori, M., Monnier, N., Daigle, N., Bathe, M., Ellenberg, J., & Lenart, P. (2011). Intracellular transport by an anchored homogeneously contracting F-actin meshwork. Current Biology, 21(7), 606-611. doi:10.1016/j.cub.2011.03.002.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-0EEE-4 Version Permalink: http://hdl.handle.net/21.11116/0000-0002-0EF3-D
Genre: Journal Article

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Mori, M., Author
Monnier, N., Author
Daigle, N., Author
Bathe, M., Author
Ellenberg, J., Author
Lenart, P.1, Author              
Affiliations:
1Research Group of Cytoskeletal Dynamics in Oocytes, MPI for Biophysical Chemistry, Max Planck Society, ou_2640691              

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 Abstract: Actin-based contractility orchestrates changes in cell shape underlying cellular functions ranging from division to migration and wound healing. Actin also functions in intracellular transport, with the prevailing view that filamentous actin (F-actin) cables serve as tracks for motor-driven transport of cargo. We recently discovered an alternate mode of intracellular transport in starfish oocytes involving a contractile F-actin meshwork that mediates chromosome congression. The mechanisms by which this meshwork contracts and translates its contractile activity into directional transport of chromosomes remained open questions. Here, we use live-cell imaging with quantitative analysis of chromosome trajectories and meshwork velocities to show that the 3D F-actin meshwork contracts homogeneously and isotropically throughout the nuclear space. Centrifugation experiments reveal that this homogeneous contraction is translated into asymmetric, directional transport by mechanical anchoring of the meshwork to the cell cortex. Finally, by injecting inert particles of different sizes, we show that this directional transport activity is size-selective and transduced to chromosomal cargo at least in part by steric trapping or "sieving." Taken together, these results reveal mechanistic design principles of a novel and potentially versatile mode of intracellular transport based on sieving by an anchored homogeneously contracting F-actin meshwork.

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Language(s): eng - English
 Dates: 2011-03-242011-04-12
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.cub.2011.03.002
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Title: Current Biology
Source Genre: Journal
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Pages: - Volume / Issue: 21 (7) Sequence Number: - Start / End Page: 606 - 611 Identifier: -