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  Monitoring the permeability of the nuclear envelope during the cell cycle.

Lenart, P., & Ellenberg, J. (2006). Monitoring the permeability of the nuclear envelope during the cell cycle. Methods, 38(1), 17-24. doi:10.1016/j.ymeth.2005.07.010.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-104F-4 Version Permalink: http://hdl.handle.net/21.11116/0000-0002-1052-F
Genre: Journal Article

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2640669.pdf (Publisher version), 400KB
 
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 Creators:
Lenart, P.1, Author              
Ellenberg, J., Author
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1Research Group of Cytoskeletal Dynamics in Oocytes, MPI for Biophysical Chemistry, Max Planck Society, ou_2640691              

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 Abstract: In animal organisms the nuclear envelope (NE) disassembles during cell division resulting in complete intermixing of cytoplasmic and nuclear compartments. This leads to the activation of many mitotic enzymes, which were kept away from their substrates or regulators by nuclear or cytoplasmic sequestration in interphase. Nuclear envelope breakdown (NEBD) is thus an essential step of mitotic entry and commits a cell to M-phase. NEBD begins with the partial disassembly of nuclear pore complexes, leading to a limited permeabilization of the NE for molecules up to ∼40 nm diameter. This is followed by the complete disruption of nuclear pores, which causes local fenestration of the double nuclear membrane and subsequently breakdown of the entire NE structure. Here, we describe the use of different sized inert fluorescent tracer molecules to directly visualize these different steps of NEBD in live cells by fluorescence microscopy.

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Language(s): eng - English
 Dates: 2005-12-152006-01
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.ymeth.2005.07.010
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Title: Methods
Source Genre: Journal
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Pages: - Volume / Issue: 38 (1) Sequence Number: - Start / End Page: 17 - 24 Identifier: -