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  Transient N-6-Methyladenosine Transcriptome Sequencing Reveals a Regulatory Role of m6A in Splicing Efficiency

Louloupi, A., Ntini, E., Conrad, T., & Ørom, U. A. (2018). Transient N-6-Methyladenosine Transcriptome Sequencing Reveals a Regulatory Role of m6A in Splicing Efficiency. Cell Reports, 23(12), 3429-3437. doi:10.1016/j.celrep.2018.05.077.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-1067-8 Version Permalink: http://hdl.handle.net/21.11116/0000-0002-1068-7
Genre: Journal Article

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 Creators:
Louloupi, Annita1, Author
Ntini, Evgenia1, Author
Conrad, Thomas1, Author              
Ørom, Ulf Andersson1, Author              
Affiliations:
1Long non-coding RNA (Ulf Andersson Ørom), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479659              

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Free keywords: RNA; RNA modifications; alternative splicing; m6A; nascent RNA; splicing dynamics
 Abstract: Splicing efficiency varies among transcripts, and tight control of splicing kinetics is crucial for coordinated gene expression. N-6-methyladenosine (m6A) is the most abundant RNA modification and is involved in regulation of RNA biogenesis and function. The impact of m6A on regulation of RNA splicing kinetics is unknown. Here, we provide a time-resolved high-resolution assessment of m6A on nascent RNA transcripts and unveil its importance for the control of RNA splicing kinetics. We find that early co-transcriptional m6A deposition near splice junctions promotes fast splicing, while m6A modifications in introns are associated with long, slowly processed introns and alternative splicing events. In conclusion, we show that early m6A deposition specifies the fate of transcripts regarding splicing kinetics and alternative splicing.

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Language(s): eng - English
 Dates: 2018-06-19
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1016/j.celrep.2018.05.077
 Degree: -

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Title: Cell Reports
Source Genre: Journal
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Publ. Info: Maryland Heights, MO : Cell Press
Pages: 9 Volume / Issue: 23 (12) Sequence Number: - Start / End Page: 3429 - 3437 Identifier: ISSN: 2211-1247
CoNE: https://pure.mpg.de/cone/journals/resource/2211-1247