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  A contractile nuclear actin network drives chromosome congression in oocytes.

Lenart, P., Bacher, C. P., Daigle, N., Hand, A. R., Eils, R., Terasaki, M., et al. (2005). A contractile nuclear actin network drives chromosome congression in oocytes. Nature, 436, 812-818.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-106C-3 Version Permalink: http://hdl.handle.net/21.11116/0000-0002-1070-D
Genre: Journal Article

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Lenart, P.1, Author              
Bacher, C. P., Author
Daigle, N., Author
Hand, A. R., Author
Eils, R., Author
Terasaki, M., Author
Ellenberg, J., Author
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1Research Group of Cytoskeletal Dynamics in Oocytes, MPI for Biophysical Chemistry, Max Planck Society, ou_2640691              

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 Abstract: Chromosome capture by microtubules is widely accepted as the universal mechanism of spindle assembly in dividing cells. However, the observed length of spindle microtubules and computer simulations of spindle assembly predict that chromosome capture is efficient in small cells, but may fail in cells with large nuclear volumes such as animal oocytes. Here we investigate chromosome congression during the first meiotic division in starfish oocytes. We show that microtubules are not sufficient for capturing chromosomes. Instead, chromosome congression requires actin polymerization. After nuclear envelope breakdown, we observe the formation of a filamentous actin mesh in the nuclear region, and find that contraction of this network delivers chromosomes to the microtubule spindle. We show that this mechanism is essential for preventing chromosome loss and aneuploidy of the egg—a leading cause of pregnancy loss and birth defects in humans.

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Language(s): eng - English
 Dates: 2005-08-11
 Publication Status: Published in print
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Title: Nature
Source Genre: Journal
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Pages: - Volume / Issue: 436 Sequence Number: - Start / End Page: 812 - 818 Identifier: -